Lysosulfatide regulates the motility of a neural precursor cell line via calcium-mediated process collapse.

Neurochemical Research
M HansJ Kappler

Abstract

Lysosulfatide is a derivative of the glycosphingolipid sulfatide. It is a major component of high density lipoproteins and was detected in the human brain. Here, we show that lysosulfatide acts as an extracellular signal regulating the migration of a neural precursor cell line (B35 neuroblastoma cells) by rapidly promoting process retraction and cell rounding. These cells express the lysosulfatide receptor S1P3 according to RT-PCR, western blotting and immunocytochemistry, but S1P3 does not mediate the effect since preincubation with three different compounds known to inhibit S1P3 did not block lysosulfatide-induced cell rounding. The signal transduction after stimulation with 3 microM lysosulfatide involves a rapid increase of [Ca2+]i which causes process retraction. This mechanism may be relevant under conditions where neural cells encounter elevated lysosulfatide levels as for example under pathological conditions after breakdown of the blood brain barrier or possibly in the lysosomal sulfatide storage disorder metachromatic leukodystrophy.

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Citations

Sep 18, 2008·Behavioural Pharmacology·Frederico R FerreiraFrancisco S Guimarães
Feb 9, 2011·Lipids in Health and Disease·Maria BlomqvistJan-Eric Månsson
May 23, 2015·Annals of Clinical and Translational Neurology·Christine Í DaliChristian Krarup
Aug 19, 2020·Essays in Biochemistry·Marco van EijkJohannes M F G Aerts
Aug 11, 2021·The Journal of Biological Chemistry·Claudia YaghootfamUlrich Matzner

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