Lysozyme-induced suppression of enzymatic and motile activities of actin-myosin: Impact of basic proteins.

International Journal of Biological Macromolecules
Masaki OkamiK Hatori

Abstract

Electrostatic interactions between actin filaments and myosin molecules, which are ubiquitous proteins in eukaryotes, are crucial for their enzymatic activity and motility. Nonspecific electrostatic interactions between proteins are unavoidable in cells; therefore, it is worth exploring how ambient proteins, such as polyelectrolytes, affect actin-myosin functions. To understand the effect of counterionic proteins on actin-myosin, we examined ATPase activity and sliding velocity via actin-myosin interactions in the presence of the basic model protein hen egg lysozyme. In an in vitro motility assay with ATP, the sliding velocity of actin filaments on heavy meromyosin (HMM) decreased with increasing lysozyme concentrations. Actin filaments were completely stalled at a lysozyme concentration above 0.08 mg/mL. Lysozyme decreased the ATP hydrolysis rate of the actin-HMM complex but not that HMM alone. Co-sedimentation assays revealed that lysozyme enhanced the binding of HMM to actin filaments in the presence of ATP. Additionally, lysozyme could bind to actin and myosin filaments. The inhibitory effect of poly-l-lysine, histone mixture, and lactoferrin on the motility of actin-myosin was higher than that of lysozyme. Thus, nonspecifi...Continue Reading

References

Mar 1, 1992·The American Journal of Physiology·E HomsherJ R Sellers
Jan 1, 1991·Methods in Enzymology·S J KronJ A Spudich
Aug 6, 1987·Nature·Y Y ToyoshimaJ A Spudich
Feb 15, 1995·The Biochemical Journal·J KołakowskiR Dabrowska
Nov 10, 2005·Biochimica Et Biophysica Acta·Brian M MartinBarbara Pliszka
Oct 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lori K SandersGerard C L Wong
Jul 8, 2010·ACS Chemical Biology·Thomas C ScanlonKarl E Griswold
Mar 18, 2011·Biophysical Chemistry·Andras MuhlradEmil Reisler
Aug 19, 2011·Advances in Protein Chemistry and Structural Biology·Rajaram SwaminathanNividh Chandra
Sep 29, 2012·Journal of Applied Physiology·Edward P DeboldMatthew A Turner
Jul 10, 2014·The Journal of Biological Chemistry·Anja M SwensonChristopher M Yengo
Feb 18, 2015·The Journal of Physical Chemistry. B·Mu YangAshutosh Tiwari
May 24, 2016·Archives of Biochemistry and Biophysics·Kuniyuki HatoriReito Wada
Oct 9, 2016·Trends in Biochemical Sciences·Anne Houdusse, H Lee Sweeney
Jul 30, 2017·Biochimica Et Biophysica Acta. General Subjects·Takahiro IwaseKuniyuki Hatori
Aug 29, 2017·PloS One·Edna BlotnickAndras Muhlrad
Sep 22, 2017·PLoS Pathogens·Stephanie A Ragland, Alison K Criss
Nov 1, 2017·Cytoskeleton·Makoto SuzukiNobuyuki Matubayasi
Dec 10, 2017·The Journal of Chemical Physics·Takato SatoMitsunori Takano
Jan 4, 2018·Cold Spring Harbor Perspectives in Biology·Tatyana Svitkina
Feb 9, 2018·Cold Spring Harbor Perspectives in Biology·H Lee Sweeney, David W Hammers
Mar 20, 2018·Biochemical and Biophysical Research Communications·Maria S Shutova, Tatyana M Svitkina
Jun 22, 2018·Nature·Christopher P CaridiIrene Chiolo
Oct 7, 2018·Biochimica Et Biophysica Acta. Proteins and Proteomics·Koji ItoKuniyuki Hatori
Oct 4, 2019·Archives of Biochemistry and Biophysics·Daria V GrigorievaAlexander V Timoshenko
Feb 12, 2020·Nature Structural & Molecular Biology·Sandro BaldiPeter B Becker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.