Lysozyme loading and release from Se doped hydroxyapatite nanoparticles

Materials Science & Engineering. C, Materials for Biological Applications
Yanhua WangShengmin Zhang

Abstract

Element-substituted hydroxyapatite (HA) based nanocomposites have become a promising therapeutic material for improving bone defect repair. Selenium substituted HA nanoparticles can both induce apoptosis of bone tumor cells and enhance osteointegration. However, the effect of selenite ions on the proteins in combination with the HA nanoparticles remains to be elucidated. Here, we investigated the influence of selenium doping concentration on the loading and release of lysozyme (LSM) as a model protein drug. The selenium substituted HA-LSM composites with different doping concentrations were synthesized and characterized. The subsequent delivery of lysozyme was studied in a phosphate buffer solution (PBS). We found that selenium substituted HA-LSM composites with Se:P=10% showed the highest amount of lysozyme loading (41.7%), whereas the amount of lysozyme loaded in undoped HA nanoparticles was the lowest (34.1%). The doped selenium interacts with lysozyme molecules, which leads to the increase of β-sheet and unordered, and the decrease of self-association, α-helix and β-turns in protein structures. Moreover, selenium addition significantly slows the protein release from HA-LSM composites. The composites with Se:P=10% release ly...Continue Reading

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Citations

Jan 17, 2017·Biological Trace Element Research·Yanhua WangShengmin Zhang
Dec 21, 2017·Chemical Society Reviews·Chao QiPeng Huang
Feb 6, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ammar Z AlshemaryZafer Evis
Mar 2, 2019·Journal of Biomedical Materials Research. Part a·Mingchen ZhouHong Bi
Jul 1, 2021·Journal of Bone and Mineral Metabolism·Tian-Lin LiHong-Guang Xu

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