Lysozyme resistance in Streptococcus suis is highly variable and multifactorial.

PloS One
Paul J Wichgers SchreurHilde E Smith

Abstract

Streptococcus suis is an important infectious agent for pigs and occasionally for humans. The host innate immune system plays a key role in preventing and eliminating S. suis infections. One important constituent of the innate immune system is the protein lysozyme, which is present in a variety of body fluids and immune cells. Lysozyme acts as a peptidoglycan degrading enzyme causing bacterial lysis. Several pathogens have developed mechanisms to evade lysozyme-mediated killing. In the present study we compared the lysozyme sensitivity of various S. suis isolates and investigated the molecular basis of lysozyme resistance for this pathogen. The lysozyme minimal inhibitory concentrations of a wide panel of S. suis isolates varied between 0.3 to 10 mg/ml. By inactivating the oatA gene in a serotype 2 and a serotype 9 strain, we showed that OatA-mediated peptidoglycan modification partly contributes to lysozyme resistance. Furthermore, inactivation of the murMN operon provided evidence that additional peptidoglycan crosslinking is not involved in lysozyme resistance in S. suis. Besides a targeted approach, we also used an unbiased approach for identifying factors involved in lysozyme resistance. Based on whole genome comparisons o...Continue Reading

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Citations

Mar 30, 2016·Forensic Science International : Synergy·E De VittoriV Romano Spica
Oct 18, 2018·Frontiers in Microbiology·David SychanthaAnthony J Clarke
Sep 29, 2020·Critical Reviews in Microbiology·Alexander V GrishinVladimir G Lunin

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Methods Mentioned

BETA
PCRs
PCR
light microscopy

Software Mentioned

CLCbio

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