Lysozyme stimulates immunoglobulin production by human-human hybridoma and human peripheral blood lymphocytes.

Cytotechnology
F MurakamiT Sugahara

Abstract

Lysozyme [EC 3.2.1.17] derived from hen egg white stimulated immunoglobulin production by human-human hybridoma, HB4C5 cells producing human lung cancer specific monoclonal IgM. IgM production by HB4C5 cells was enhanced more than 13-fold by the addition of lysozyme at 380 μg/ml in a serum-free medium. The immunoglobulin production stimulating effect of lysozyme was observed immediately after inoculation and maintained for 5 days. Lysozyme enhanced immunoglobulin production by the hybridoma line without growth promotion. This enzyme also accelerated IgM and IgG production of human peripheral blood lymphocytes 5.3-fold and 2.3-fold, respectively. These results suggest that lysozyme stimulates immunoglobuling production of not only specific hybridoma line, but also non-specific immunoglobulin producers. However, although the enzymatic activity of lysozyme was almost lost by heat-treatment at 100 °C for 30 min, the IPSF activity was retained. This fact suggests that IPSF activity of lysozyme does not come from its enzymatic activity or reaction products. All these findings clearly indicate that lysozyme has a novel function as an immunoglobulin production stimulating factor. GAPDH - glyceraldehyde-3-phosphate dehydrogenase; Ig - i...Continue Reading

References

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Citations

Jul 21, 2011·Cytotechnology·Hitoshi MorishigeMikiharu Doi
May 12, 2000·Biochimica Et Biophysica Acta·T SugaharaT Sasaki
Sep 3, 2002·Biochimica Et Biophysica Acta·Takuya SugaharaTakeshi Sasaki
Nov 13, 2008·Cancer Biotherapy & Radiopharmaceuticals·Ali I GuzelAyfer Pazarbasi
Aug 7, 2002·Bioscience, Biotechnology, and Biochemistry·Takeaki OkamotoKoji Yamada
Apr 23, 2011·Bioscience, Biotechnology, and Biochemistry·Miho DaifukuTakuya Sugahara
Jan 14, 2011·Bioscience, Biotechnology, and Biochemistry·Kosuke NishiTakuya Sugahara
Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Deniz Türkmen, Adil Denizli
Sep 9, 2006·Bioscience, Biotechnology, and Biochemistry·Takuya SugaharaSatoshi Yamauchi
Jan 3, 2015·Journal of Biomaterials Science. Polymer Edition·Murat UygunAdil Denizli
Mar 3, 2018·Cytotechnology·Ayuka TagashiraTakuya Sugahara
Mar 11, 2000·Biotechnology and Bioengineering·R Ghosh, Z F Cui
Nov 1, 2012·Artificial Cells, Nanomedicine, and Biotechnology·Kazım Köse, Adil Denizli
Jan 14, 2021·Chemical Communications : Chem Comm·Cagla SahinMichael Landreh
May 30, 2021·International Journal of Biological Macromolecules·Pankaj AttriAnnemie Bogaerts
Mar 7, 2006·International Journal of Biological Macromolecules·Evrim Banu Altintaş, Adil Denizli

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