PMID: 18424602Apr 22, 2008Paper

Lysozyme transgenic goats' milk influences gastrointestinal morphology in young pigs

The Journal of Nutrition
Dottie R BrundigeJames D Murray

Abstract

Transgenesis provides a method of expressing novel proteins in milk to increase the functional benefits of milk consumption. Transgenic goats expressing human lysozyme (hLZ) at 67% of the concentration in human breast milk were produced, thereby enhancing the antimicrobial properties of goats' milk. The objective of this study was to investigate the impact of pasteurized milk containing hLZ on growth, the intestinal epithelium, and an enteropathogenic Escherichia coli (EPEC) infection in young weaned pigs. Pigs were placed into 4 groups and fed a diet of solid food and either control (nontransgenic) goats' milk or milk from hLZ-transgenic goats. Growth was assessed by weight gain. Nonchallenged pigs were necropsied after 6 wk, whereas the remaining pigs were necropsied at 7 wk following bacterial challenge. We determined the numbers of total coliforms and E. coli and examined small intestinal histology for all pigs. Complete blood counts were also determined pre- and postchallenge. Challenged pigs receiving hLZ milk had fewer total coliforms (P = 0.029) and E. coli (P = 0.030) in the ileum than controls. hLZ-fed pigs also had a greater duodenal villi width (P = 0.029) than controls. Additionally, nonchallenged hLZ-fed pigs had ...Continue Reading

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Citations

Nov 6, 2009·Transgenic Research·James D Murray, Elizabeth A Maga
Mar 17, 2010·Journal of Animal Science·S C FahrenkrugB P Glenn
Jun 16, 2010·Transgenic Research·Jia TongNing Li
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Feb 12, 2011·Transgenic Research·Caitlin A CooperJames D Murray
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Sep 2, 2021·Mucosal Immunology·Kristen L Lokken-ToyliJeffrey N Weiser

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