Lyssavirus Vaccine with a Chimeric Glycoprotein Protects across Phylogroups.

Cell Reports
Christine R FisherMatthias J Schnell

Abstract

Rabies is nearly 100% lethal in the absence of treatment, killing an estimated 59,000 people annually. Vaccines and biologics are highly efficacious when administered properly. Sixteen rabies-related viruses (lyssaviruses) are similarly lethal, but some are divergent enough to evade protection from current vaccines and biologics, which are based only on the classical rabies virus (RABV). Here we present the development and characterization of LyssaVax, a vaccine featuring a structurally designed, functional chimeric glycoprotein (G) containing immunologically important domains from both RABV G and the highly divergent Mokola virus (MOKV) G. LyssaVax elicits high titers of antibodies specific to both RABV and MOKV Gs in mice. Immune sera also neutralize a range of wild-type lyssaviruses across the major phylogroups. LyssaVax-immunized mice are protected against challenge with recombinant RABV and MOKV. Altogether, LyssaVax demonstrates the utility of structural modeling in vaccine design and constitutes a broadened lyssavirus vaccine candidate.

References

Aug 1, 1991·Journal of Virology·A BenmansourF Lafay
Nov 1, 1970·Journal of Virology·R E ShopeD L Moore
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B DietzscholdH Koprowski
Dec 16, 1998·Journal of Virology·C JalletP Perrin
Oct 22, 2002·The Journal of General Virology·N JohnsonA R Fooks
Apr 5, 2005·Journal of Neurovirology·Monique Lafon
May 18, 2005·Virus Research·Cathleen A HanlonCharles E Rupprecht
Feb 10, 2007·Science·Stéphane RocheStéphane Bressanelli
Mar 21, 2007·Emerging Infectious Diseases·Macpherson MallewaTom Solomon
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Feb 7, 2008·Emerging Infectious Diseases·Claude T SabetaLouis H Nel
Feb 29, 2008·Journal of Clinical Microbiology·Ivan V KuzminCharles E Rupprecht
Oct 13, 2009·FEMS Immunology and Medical Microbiology·Claude SabetaAlex Wandeler
Dec 25, 2009·Emerging Infectious Diseases·Sally J CutlerWim H M van der Poel
Jan 8, 2010·Journal of Virology·Emily A GommeMatthias J Schnell
May 12, 2010·Biochemical and Biophysical Research Communications·Jian-chao WeiPu-yan Chen
Sep 10, 2010·Journal of Virology·D L HortonD J Smith
Sep 14, 2010·Vaccine·UNKNOWN WHO Publication
Apr 4, 2012·Emerging Infectious Diseases·Denise A MarstonTiziana Lembo
May 1, 2012·The Lancet Infectious Diseases·Leonard BothAnthony R Fooks
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona

❮ Previous
Next ❯

Citations

Apr 4, 2021·Viruses·Jessica CoertseWanda Markotter

❮ Previous
Next ❯

Methods Mentioned

BETA
Infection
blood draw
enzyme-linked immunosorbent assay
ELISA
Assay
glycosylation
ELISAs
PCR
transfection

Software Mentioned

XtremeGene
Jenoptic
Phyre2
MODEL
SWISS
GraphPad Prism
ProgRes
Fiji
TASSER

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.