Lysyl hydroxylase-2b directs collagen cross-linking pathways in MC3T3-E1 cells

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Suchaya PornprasertsukMitsuo Yamauchi

Abstract

To elucidate the roles of LH2b in collagen cross-linking, MC3T3-E1 cell clones expressing higher (S) or lower (AS) levels of LH2b were established. Compared with controls, the collagen cross-linking pattern was shifted toward hydroxylysine-aldehyde (S clones)- or lysine-aldehyde (AS clones)-derived pathways. The data indicate that LH2b directs collagen cross-linking pathways through its action on telopeptidyl lysine residues. Lysine (Lys) hydroxylation is a post-translational modification of collagen critical for cross-linking and glycosylation. Currently, three isoforms of lysyl hydroxylase (LH) have been identified, but their specific functions are still not well defined. Recently, we proposed that LH2 might modulate collagen cross-linking pattern through its action on Lys residues located in the telopeptide domains of collagen. To directly test this hypothesis, several MC3T3-E1 cell-derived clones expressing higher (sense [S]) or lower (antisense [AS]) levels of LH2b, the predominant form of LH2 in this cell line, were established and cultured for 2 weeks, and collagen cross-links and precursor aldehydes in the matrices were analyzed. In S clones tested, the ratio of dihydroxylysinonorleucine (DHLNL) to hydroxylysinonorleuci...Continue Reading

References

Jan 1, 1985·Annals of the New York Academy of Sciences·K I Kivirikko, R Myllylä
Jan 1, 1984·Annual Review of Biochemistry·D R EyreP M Gallop
Oct 17, 1995·Biochimica Et Biophysica Acta·L C Armstrong, J A Last
Jan 1, 1993·Connective Tissue Research·M Yamauchi, E P Katz
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·K PassojaK I Kivirikko
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D WangR T Franceschi
Jun 18, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·H N Yeowell, L C Walker
Aug 3, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·H RuotsalainenR Myllylä
Aug 24, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K UzawaM Yamauchi
Jul 14, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R A BankR J Sakkers
May 4, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·H RuotsalainenR Myllylä
Jul 25, 2003·Biochemical and Biophysical Research Communications·Derry K MercerSimon P Robins
Jul 26, 2003·The Journal of Biological Chemistry·Annemarie J van der SlotRuud A Bank

❮ Previous
Next ❯

Citations

Nov 7, 2013·Cell and Tissue Research·Dennis F G RemstPeter M van der Kraan
Jan 19, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Maryna PerepelyukRebecca G Wells
Dec 28, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Suchaya PornprasertsukMitsuo Yamauchi
Dec 13, 2005·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·S Viguet-CarrinP D Delmas
Sep 26, 2013·Diseases of Aquatic Organisms·Mona E PedersenKirsten O Hannesson
Apr 25, 2007·Connective Tissue Research·Michitsuna KatafuchiMitsuo Yamauchi
Jan 17, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Philip C Trackman
Dec 4, 2008·Matrix Biology : Journal of the International Society for Matrix Biology·Yoshiyuki MochidaMitsuo Yamauchi
Oct 28, 2008·Burns : Journal of the International Society for Burn Injuries·Willem M van der VeerFrank B Niessen
Mar 2, 2016·The Canadian Journal of Cardiology·Birgit KahleJürgen Brinckmann
Feb 10, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Kati TakaluomaJohanna Myllyharju
Jul 21, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Antoon J van den BogaerdtMagda M W Ulrich
Sep 17, 2005·Journal of Cellular Biochemistry·Philip C Trackman
Oct 15, 2013·Clinical Oral Implants Research·Ghadeer N ThaljiLyndon F Cooper
May 9, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hideaki NagaokaMitsuo Yamauchi
Jan 18, 2005·Biochemical and Biophysical Research Communications·Phimon AtsawasuwanMitsuo Yamauchi
Oct 22, 2008·Biochemical and Biophysical Research Communications·Hideaki NagaokaMitsuo Yamauchi
Aug 29, 2006·Experimental Cell Research·Jiang WuJürgen Brinckmann
Oct 15, 2014·Journal of Prosthodontic Research·Masaru Kaku, Mitsuo Yamauchi
Aug 21, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hiroshi TsukamotoYoshiaki Akahane
Jun 20, 2012·Essays in Biochemistry·Mitsuo Yamauchi, Marnisa Sricholpech
Jun 16, 2011·Biochemical and Biophysical Research Communications·Yoshiyuki MochidaMitsuo Yamauchi
Oct 18, 2016·International Journal of Implant Dentistry·Siddharth ShanbhagAndreas Stavropoulos
May 10, 2011·Bone·Daniela B S MendonçaLyndon F Cooper
May 10, 2005·Biochimica Et Biophysica Acta·Annemarie J van der Slot-VerhoevenRuud A Bank
Jun 16, 2005·Biochimica Et Biophysica Acta·Annemarie J van der SlotAnne-Marie Zuurmond
Aug 17, 2010·Biochemical and Biophysical Research Communications·F VargaK Klaushofer
Jun 8, 2007·Biochemical and Biophysical Research Communications·Masaru KakuMitsuo Yamauchi
Feb 11, 2015·The Journal of Clinical Investigation·Yulong ChenJonathan M Kurie
Jun 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Rutger A F GjaltemaRuud A Bank
Dec 23, 2016·Critical Reviews in Biochemistry and Molecular Biology·Rutger A F Gjaltema, Ruud A Bank
Dec 29, 2012·Cartilage·Terajima MasahikoNancy Pleshko
Feb 22, 2017·Archives of Biochemistry and Biophysics·Hou-Fu GuoJonathan M Kurie
Jul 12, 2017·Journal of Proteome Research·Masahiko TerajimaMitsuo Yamauchi
Feb 23, 2019·Journal of Dental Research·T SaitoM Yamauchi
Apr 5, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ivana MižíkováRory E Morty
Jun 8, 2019·PLoS Genetics·Masahiko TerajimaMitsuo Yamauchi
Jan 12, 2011·The Journal of Biological Chemistry·Marnisa SricholpechMitsuo Yamauchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Suchaya PornprasertsukMitsuo Yamauchi
Biochemical and Biophysical Research Communications
Derry K MercerSimon P Robins
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Katsuhiro UzawaM Yamauchi
Matrix Biology : Journal of the International Society for Matrix Biology
Annemarie J van der SlotRuud A Bank
© 2022 Meta ULC. All rights reserved