Lysyl hydroxylase 3-mediated glucosylation in type I collagen: molecular loci and biological significance.

The Journal of Biological Chemistry
Marnisa SricholpechMitsuo Yamauchi

Abstract

Recently, by employing the short hairpin RNA technology, we have generated MC3T3-E1 (MC)-derived clones stably suppressing lysyl hydroxylase 3 (LH3) (short hairpin (Sh) clones) and demonstrated the LH3 function as glucosyltransferase in type I collagen (Sricholpech, M., Perdivara, I., Nagaoka, H., Yokoyama, M., Tomer, K. B., and Yamauchi, M. (2011) Lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen in osteoblast culture. J. Biol. Chem. 286, 8846-8856). To further elucidate the biological significance of this modification, we characterized and compared type I collagen phenotypes produced by Sh clones and two control groups, MC and those transfected with empty vector. Mass spectrometric analysis identified five glycosylation sites in type I collagen (i.e. α1,2-87, α1,2-174, and α2-219. Of these, the predominant glycosylation site was α1-87, one of the major helical cross-linking sites. In Sh collagen, the abundance of glucosylgalactosylhydroxylysine was significantly decreased at all of the five sites with a concomitant increase in galactosylhydroxylysine at four of these sites. The collagen cross-links were significantly diminished in Sh clones, and, for the major cross-link, dihydroxylysinonorl...Continue Reading

References

Jan 1, 1976·Advances in Experimental Medicine and Biology·R C SiegelY Chang
Jan 1, 1976·International Review of Connective Tissue Research·P P Fietzek, K Kühn
Jan 1, 1979·International Review of Connective Tissue Research·K I Kivirikko, R Myllylä
Jun 24, 1977·Biochimica Et Biophysica Acta·S P Robins, A J Bailey
Feb 1, 1990·European Journal of Clinical Investigation·R E BrennerP K Müller
Jan 1, 1985·Connective Tissue Research·A L CronlundH M Kagan
Jan 1, 1987·Methods in Enzymology·D Eyre
Aug 1, 1987·Collagen and Related Research·S AmudeswariB Chakrabarti
May 15, 1973·Biochemical and Biophysical Research Communications·D R Eyre, M J Glimcher
Dec 6, 1973·Biochimica Et Biophysica Acta·A J BaileyA Delaunay
Jan 19, 1971·Biochimica Et Biophysica Acta·S R PinnellS M Krane
Aug 1, 1970·The Journal of Clinical Investigation·J P Segrest, L W Cunningham
Jan 1, 1982·Methods in Enzymology·K I Kivirikko, R Myllylä
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M YamauchiG L Mechanic
Jan 29, 1980·Biochemical and Biophysical Research Communications·D R Eyre, H Oguchi
Jan 1, 1995·Journal of Cancer Research and Clinical Oncology·H W LehmannP K Müller
Jan 1, 1993·Connective Tissue Research·M Yamauchi, E P Katz
May 14, 1993·Biochemical and Biophysical Research Communications·M MichalskyL Moro
Oct 25, 1996·The Journal of Biological Chemistry·D A Hanson, D R Eyre
Jan 1, 1997·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·L MoroK N Suarez
Apr 1, 1997·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·L MoroM Romanello
Jan 12, 1999·Mechanisms of Ageing and Development·A J BaileyL Knott
Aug 10, 2000·The Journal of Biological Chemistry·J HeikkinenR Myllylä
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 12, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Chunguang WangRaili Myllylä

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Oct 1, 2015·Annual Review of Cell and Developmental Biology·Vivek Malhotra, Patrik Erlmann
Apr 4, 2014·Acta Odontologica Scandinavica·Takashi MatsuuraHironobu Sato
Dec 23, 2016·Critical Reviews in Biochemistry and Molecular Biology·Rutger A F Gjaltema, Ruud A Bank
Nov 17, 2015·The Analyst·H J SimonP B O'Connor
Jul 12, 2017·Journal of Proteome Research·Masahiko TerajimaMitsuo Yamauchi
Feb 8, 2018·Nature Communications·Hou-Fu GuoJonathan M Kurie
Oct 10, 2018·Chemical Communications : Chem Comm·Wing Ying ChowCatherine M Shanahan
Jul 21, 2016·Nature Communications·Blerida BanushiPaul Gissen
Aug 21, 2018·Advanced Materials·Anna SorushanovaDimitrios I Zeugolis
Jul 18, 2013·Australian Journal of Chemistry·Irina PerdivaraKenneth B Tomer
May 9, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hideaki NagaokaMitsuo Yamauchi
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