Lysyl oxidase expression in a rat model of arterial balloon injury

Journal of Vascular Surgery
Vijay K NuthakkiCharles J Shanley

Abstract

Traditional therapies for arteriosclerotic disease often fail as a result of an exaggerated fibroproliferative response (recurrent stenosis) at the site of the intervention. Lysyl oxidase, secreted by activated vascular smooth muscle cells and fibroblasts, catalyzes a key step in the cross-linking and stabilization of collagen and elastin in the vascular wall. We hypothesized that lysyl oxidase messenger RNA (mRNA) and protein expression are time-dependent and precede collagen accumulation and luminal narrowing after arterial balloon injury in the rat. A 2F balloon-tipped catheter was used to injure the right common carotid artery in male Sprague-Dawley rats. Injured right and control (uninjured) left common carotid arteries were harvested at 0, 0.25, 1, 3, 7, 14, 21, 28, and 60 days for mRNA quantitation and immunohistochemical analysis. Steady-state lysyl oxidase mRNA levels were quantitated with real-time reverse transcription polymerase chain reaction (TaqMan). Immunohistochemical staining with antibodies to alpha-smooth muscle cell actin and lysyl oxidase, and Movat pentachrome staining were performed for qualitative assessment of changes in the cellular and extracellular matrix components of the vessel wall. Post-injury i...Continue Reading

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Citations

May 13, 2008·Cardiovascular Research·Cristina RodríguezLina Badimon
Mar 21, 2009·Journal of Applied Physiology·Eric C TudayDan E Berkowitz
Jul 25, 2013·Annals of Vascular Surgery·Yevgeniy RitsCharles J Shanley
Jul 6, 2014·Thrombosis and Haemostasis·Mar OrriolsCristina Rodríguez
Oct 3, 2006·Journal of Vascular Surgery·Deborah AbelesGuillermo García-Cardeña
Mar 19, 2016·Blood·Diane S Krause
Mar 29, 2005·American Journal of Surgery·Amy R WoznickCharles J Shanley
Jul 15, 2006·Trends in Cardiovascular Medicine·Richard D KenagyThomas N Wight
Oct 24, 2007·Journal of Vascular Surgery·Seung-Kee MinAlexander W Clowes
Dec 7, 2007·Biochemical and Biophysical Research Communications·Paola A HurtadoPhilip C Trackman
Sep 30, 2008·Basic Research in Cardiology·Wei-Jan ChenSu-Hui Yang
Jan 26, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Takashi KohnoTohru Fukai
Jun 14, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Camille BrasseletAntoine Lafont
Jul 20, 2007·Physiological Genomics·Utako YokoyamaSusumu Minamisawa
Oct 17, 2019·Biomolecules·José Martínez-GonzálezCristina Rodríguez
Jan 17, 2017·Tissue Engineering and Regenerative Medicine·Linyi CaiJing Xie

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