Lyt phenotypes of primary cytotoxic T cells generated across the A and E region of the H-2 complex

European Journal of Immunology
D VidovicJ Klein

Abstract

Six different cell-mediated lympholysis (CML) combinations were established, four of which generated effector cells against the I-E, and two against the I-A molecule. The cell surface phenotype of effector cells was then determined by depletion of cytotoxic T lymphocyte (CTL) activity with antisera and rabbit complement (C) treatment. Both types of effector cells were completely eliminated by treatment with anti-Thy-1.2 antiserum plus C. Anti-Lyt-1.2 and C depleted anti-A and anti-E killer activity but did not eliminate CTL generated across a whole H-2 difference. One out of three different batches of anti-Lyt-2.2 antiserum did not deplete anti-A killer activity, while it efficiently eliminated CTL generated across the E region or whole H-2 difference. However, two batches of anti-Lyt-2.2 antiserum depleted also anti-A CTL activity. A quantitative difference between anti-A and anti-E CTL in terms of Lyt-2 expression was demonstrated by significant differences in recovery of killer activity, after treatment of these two types of CTL with a wide concentration range of the same anti-Lyt-2.2 antiserum and C. Thus it is concluded that anti-A killer cells have the cell surface phenotype of Thy-1+, Lyt-1, Lyt-2, whereas anti-E CTL are...Continue Reading

References

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Citations

Jan 1, 1982·Immunogenetics·C MarkJ Klein
Jan 1, 1992·Biotherapy·D W Lancki, F W Fitch
Jun 1, 1982·The Journal of Experimental Medicine·C Spellman, R E Anderson
Apr 1, 1986·The Journal of Experimental Medicine·N ShinoharaD H Sachs
Jan 11, 2012·Scandinavian Journal of Immunology·Z A Nagy
Sep 1, 1983·Immunology Today·J A Bluestone, R J Hodes
Jan 1, 1983·Immunological Reviews·S L Swain
Jul 1, 1988·International Journal of Dermatology·T ShioharaM Nagashima
Aug 1, 1986·European Journal of Immunology·C N BaxevanisM Papamichail
Jun 1, 1985·Cell Biophysics·T N Buican, G W Hoffmann
Apr 1, 1984·European Journal of Immunology·W Haas, H von Boehmer

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