Lytic action of beta-(1-3)-glucanase on yeast cells.

Journal of Bacteriology
J MonrealJ R Villanueva

Abstract

Candida utilis, Saccharomyces cerevisiae, S. fragilis, Pichia polymorpha, and Hansenula anomala yeast cells, harvested in the early logarithmic phase, were attacked with purified beta(1-3)-glucanase from Micromonospora chalcea, which resulted in the liberation of protoplasts. The treated cells were observed under the electron microscope before the protoplasts were liberated. Differences in the cell walls of the enzyme-treated and untreated cells were observed. The action of the glucanase was also tested against isolated walls of C. utilis. The enzyme attacked the S. cerevisiae cell wall in a uniform manner. The attack on S. fragilis was located in certain zones of the cell wall, where breakage occurred and through which the protoplast emerged. On the other three yeasts, an intermediate attack was observed, not as definitely located as in S. fragilis, yet less uniformly than in S. cerevisiae.

References

Oct 20, 1966·Biochemical and Biophysical Research Communications·S NagasakiJ O Lampen
Mar 1, 1961·The Journal of Biophysical and Biochemical Cytology·E VITOLSA W LINNANE
Sep 1, 1964·Journal of Bacteriology·G S KOBAYASHIJ F KOFROTH
Jan 22, 1965·Archiv für Mikrobiologie·R SENTANDREU, J R VILLANUEVA
May 1, 1965·Canadian Journal of Biochemistry·H M HEICK, H B STEWART

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Citations

Jan 1, 1971·Archiv für Mikrobiologie·J H Sietsma, J T Wouters
Jan 1, 1977·Journal of Bacteriology·T SantosC Nombela

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