Lytic activity of LysH5 endolysin secreted by Lactococcus lactis using the secretion signal sequence of bacteriocin Lcn972.

Applied and Environmental Microbiology
Lorena Rodríguez-RubioPilar García

Abstract

Bacteriophage endolysins have an interesting potential as antimicrobials. The endolysin LysH5, encoded by Staphylococcus aureus phage vB_SauS-phi-IPLA88, was expressed and secreted in Lactococcus lactis using the signal peptide of bacteriocin lactococcin 972 and lactococcal constitutive and inducible promoters. Up to 80 U/mg of extracellular active endolysin was detected in culture supernatants, but most of the protein (up to 323 U/mg) remained in the cell extracts.

References

Oct 1, 1996·Applied and Environmental Microbiology·P G de RuyterW M de Vos
Aug 3, 2001·International Journal of Food Microbiology·M L De BuyserV Lafarge
Aug 30, 2001·Applied and Environmental Microbiology·Y Le LoirP Langella
Feb 15, 2003·Biochimica Et Biophysica Acta·Edmund R S KunjiBert Poolman
Jun 18, 2003·International Journal of Food Microbiology·Natalia RillaAna Rodríguez
Jan 12, 2005·Protein Expression and Purification·Michael W LairdRobert N Burke
Aug 10, 2005·Applied Microbiology and Biotechnology·Igor Mierau, Michiel Kleerebezem
Nov 7, 2006·Applied and Environmental Microbiology·Peter Sass, Gabriele Bierbaum
Oct 25, 2007·Journal of Molecular Microbiology and Biotechnology·E MorelloI Poquet
Sep 24, 2008·International Journal of Food Microbiology·José María ObesoPilar García
Oct 20, 2009·Applied and Environmental Microbiology·Pilar GarcíaAna Rodríguez
Mar 17, 2010·Applied and Environmental Microbiology·Régis StentzClaire Shearman
May 11, 2010·International Journal of Medical Microbiology : IJMM·Vincent A Fischetti
Jun 12, 2010·International Journal of Food Microbiology·Pilar GarcíaAna Rodríguez
Oct 22, 2010·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·R KöckA W Friedrich
Mar 4, 2011·Journal of Veterinary Science·Manuela OliveiraCristina Lobo Vilela

❮ Previous
Next ❯

Citations

Oct 19, 2012·Applied Microbiology and Biotechnology·Piotr SzwedaSlawomir Milewski
Dec 29, 2015·Current Opinion in Biotechnology·Mathias Schmelcher, Martin J Loessner
Jan 28, 2014·Letters in Applied Microbiology·M H Farris, A D Steinberg
Apr 20, 2016·Frontiers in Microbiology·Jaewoo BaiJu-Hoon Lee
Jan 22, 2015·Critical Reviews in Biotechnology·Lorena Rodríguez-RubioPilar García
Jul 5, 2016·Frontiers in Microbiology·Diana GutiérrezPilar García
Feb 8, 2013·Journal of Veterinary Science·Anil Kumar MishraManjunatha Reddy
Jul 26, 2017·Science Progress·Jude AjueborAidan Coffey
Dec 24, 2019·Frontiers in Bioengineering and Biotechnology·Laura StoffelsBrenda Parker
Jul 3, 2013·ACS Synthetic Biology·Katherine VolzingYiannis N Kaznessis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.