Abstract
The protein kinase MELK is implicated in the control of cell proliferation, cell cycle and mRNA splicing. We previously showed that MELK activity is correlated with its phosphorylation level, is cell cycle dependent, and maximal during mitosis. Here we report on the identification of T414, T449, T451, T481 and S498 as residues phosphorylated in Xenopus MELK (xMELK) in M-phase egg extract. Phosphorylations of T449, T451, T481 are specifically detected during mitosis. Results obtained in vivo showed that MPF and MAPK pathways are involved in xMELK phosphorylation. In vitro, MPF and MAPK directly phosphorylate xMELK and MPF phosphorylates xMELK on T481. In addition, phosphorylation by MPF and MAPK enhances MELK activity in vitro. Taken together our results indicate that MELK phosphorylation by MPF and MAPK enhance its activity during M-phase.
Citations
Sep 5, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ruprecht KunerHolger Sültmann
Apr 26, 2012·The Journal of Biological Chemistry·Hyun-A Seong, Hyunjung Ha
Nov 5, 2013·International Journal of Molecular Sciences·Pengfei Jiang, Deli Zhang
May 12, 2011·Neuro-oncology·Ichiro NakanoHarley I Kornblum
Apr 28, 2010·Experimental Cell Research·Caroline BadouelJean-Pierre Tassan
Mar 15, 2016·Proteome Science·Suely Kazue Nagahashi MarieMiyuki Uno
Oct 26, 2007·International Journal of Cancer. Journal International Du Cancer·Suely K N MarieOtavia L Caballero
Jan 23, 2013·Stem Cells·Chunyu GuIchiro Nakano
May 24, 2011·Reproduction in Domestic Animals = Zuchthygiene·C C HsuS T Ding
Jun 3, 2014·Molecular Cancer·Tao DuBingya Liu
Aug 3, 2011·Biochemical and Biophysical Research Communications·Seungho Choi, Ja-Lok Ku
Nov 30, 2006·Cell Biology International·Isabelle ChartrainJean-Pierre Tassan
Apr 16, 2016·PloS One·Wenbin JiSong-Tao Liu
Jul 10, 2013·The Journal of Biological Chemistry·Cenk KigMathieu Bollen
Nov 17, 2017·Scientific Reports·Elizabeth H PeuchenPaul W Huber
Feb 14, 2018·EMBO Molecular Medicine·Sarah JurmeisterDavid E Neal
Dec 22, 2019·Medicina·Antonio CiglianoKirsten Utpatel
Feb 9, 2018·Oncotarget·Shan GuanJianhua Yang
Dec 10, 2017·The Journal of Biological Chemistry·Xiao-Man LiuCai-Xia Yang
Nov 12, 2019·Neuro-oncology·Dean Popovski, Annie Huang
Oct 4, 2015·Bioscience Reports·Lijs BekeMathieu Bollen
Aug 3, 2017·Expert Opinion on Therapeutic Targets·Mary Kathryn PitnerChandra Bartholomeusz
Sep 11, 2019·Neuro-oncology·Michaël H MeelEsther Hulleman
Aug 1, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michaël H MeelEsther Hulleman
Oct 30, 2013·Biology Open·Isabelle ChartrainJean-Pierre Tassan
Mar 8, 2011·Journal of Cell Science·Yann Le PageJean-Pierre Tassan
Sep 23, 2021·G3 : Genes - Genomes - Genetics·Lin ZhouFloris Foijer