M protein of the group A Streptococcus binds to the seventh short consensus repeat of human complement factor H.

Infection and Immunity
T K BlackmoreDavid L Gordon

Abstract

Streptococcus pyogenes evades complement by binding the complement-regulatory protein factor H (fH) via the central conserved C-repeat region of M protein. However, the corresponding binding region within fH has not previously been precisely localized. fH is composed of 20 conserved modules called short consensus repeats (SCRs), each of which contains approximately 60 amino acids. A series of fH truncated and deletion mutants were prepared, and their interaction with M6 protein was examined. The M protein binding site was initially localized to SCRs 6 to 15 as demonstrated by ligand dot blotting, chemical cross-linking, and enzyme-linked immunosorbent assay. SCR 7 was then shown to contain the M protein binding site, as a construct consisting of the first seven SCRs bound M protein but a construct containing the first six SCRs did not bind. In addition, deletion of SCR 7 from full-length fH abolished binding to M protein. SCR 7 is known to contain a heparin binding domain, and binding of fH to M6 protein was almost totally inhibited in the presence of 400 U of heparin per ml. These results localize the M6 protein binding site of fH to SCR 7 and indicate that it is in close proximity to the heparin binding site.

References

Nov 2, 1976·The Journal of Experimental Medicine·K Whaley, S Ruddy
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D T Fearon
May 1, 1979·The Journal of Infectious Diseases·P K PetersonP G Quie
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J M WeilerD T Fearon
Dec 1, 1992·Infection and Immunity·R D HorstmannV A Fischetti
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S Meri, M K Pangburn
Mar 1, 1986·The Journal of Infectious Diseases·D L Kasper
Mar 1, 1985·The Journal of Experimental Medicine·K F JonesV A Fischetti
Jan 1, 1989·Advances in Immunology·D HourcadeJ P Atkinson
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R D HorstmannV A Fischetti
Nov 1, 1969·The Journal of Experimental Medicine·J SwansonE C Gotschlich
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G N PhillipsV A Fischetti
Mar 1, 1995·Molecular Microbiology·J Perez-CasalJ R Scott
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N OkadaM Caparon
Jan 1, 1995·Infection and Immunity·V A FischettiV Pancholi
Jan 25, 1996·The New England Journal of Medicine·A L Bisno, D L Stevens
Mar 29, 1997·Lancet·G H Stollerman

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Citations

Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eleni GiannakisDavid L Gordon
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael K Pangburn
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer L McRaeDavid L Gordon
Dec 22, 2007·Seminars in Ophthalmology·Sandra R MontezumaJohanna M Seddon
Jan 1, 2008·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Madeleine ZetterbergHenrik Zetterberg
Dec 18, 2007·Clinical and Experimental Immunology·C Q SchmidtP N Barlow
Dec 6, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karita HaapasaloT Sakari Jokiranta
Jan 29, 2002·The Journal of Biological Chemistry·Thomas AreschougGunnar Lindahl
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuko KimuraTsukasa Seya
Apr 2, 2002·Journal of Cardiac Surgery·S MiyagawaR Shirakura
Apr 7, 2006·The Journal of Biological Chemistry·Ling LuJing-Ren Zhang
Mar 24, 2005·Infection and Immunity·Marta Biedzka-SarekMikael Skurnik
Jul 16, 2005·Ophthalmic Genetics·Susan L SantangeloJohanna M Seddon
Jul 18, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ravi MaruvadaC E Rubens
May 3, 2013·PLoS Pathogens·T MeriT S Jokiranta
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanna JarvaSeppo Meri
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sandhya DaveLarry S McDaniel
Jul 31, 2007·Human Genetics·Andrew Lotery, Dorothy Trump
Mar 1, 2014·Emerging Microbes & Infections·Xiaolei PanJing-Ren Zhang
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanna JarvaSeppo Meri
Nov 1, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna M BlomDavid Ermert
Nov 24, 2019·Seminars in Immunology·Marcell CserhalmiMihály Józsi
Sep 5, 2017·Trends in Microbiology·Partho Ghosh
Nov 24, 1999·Biochemical and Biophysical Research Communications·M YoshitatsuR Shirakura

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