M-Type Pyruvate Kinase Isoforms and Lactate Dehydrogenase A in the Mammalian Retina: Metabolic Implications

Investigative Ophthalmology & Visual Science
Robert J CassonGlyn Chidlow

Abstract

Like cancer cells, photoreceptor cells produce lactate aerobically, requiring lactate dehydrogenase A (LDH-A). Cancer cells also use glycolytic intermediates for biosynthesis. The molecular switch controlling glycolytic flow is thought to be an isoenzyme of pyruvate kinase (PKM2). Here, we determined the expression and localization of PKM2 and LDH-A in mammalian retina and make comparisons with the brain. Single- and double-labeling immunohistochemistry for PKM2, pyruvate kinase M1 (PKM1), and LDH-A were performed using retinal sections from C57BL/6 mice, Sprague-Dawley rats, rabbits, marmosets, and humans. Pyruvate kinase M1 and PKM2 mRNA and protein expression levels were quantified in rodent retina and brain by using qPCR and immunoblotting. The quaternary forms of PKM2 in rat retina were also determined. Pyruvate kinase M2 was present in some glial cells and rod and cone photoreceptors in the retina of all species but was exclusively localized to glia in the brain. Pyruvate kinase M1 was confined to neurons in the retina and brain. Lactate dehydrogenase A was principally found in photoreceptors and inner portion of the avascular rabbit retina. Western blotting and qPCR confirmed high levels of PKM2 and LDH-A in the retina. ...Continue Reading

Citations

Aug 21, 2016·Cellular and Molecular Life Sciences : CMLS·Thierry Léveillard, José-Alain Sahel
Mar 24, 2017·Clinical & Experimental Ophthalmology·Daniel S NarayanRobert J Casson
Jun 10, 2017·ELife·Yashodhan ChinchoreConstance L Cepko
Dec 7, 2017·Scientific Reports·Sara BarbatoIvan Conte
Jul 28, 2020·Clinical & Experimental Ophthalmology·Cameron D HaydingerDaniel J Peet
Jul 7, 2017·ELife·James B Hurley
Dec 6, 2019·Frontiers in Neuroscience·Daniel S NarayanRobert J Casson
Sep 9, 2017·Frontiers in Neuroscience·Glyn ChidlowRobert J Casson
Feb 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Allison GrenellJianhai Du
Jul 10, 2017·Journal of Molecular Neuroscience : MN·Alexandre ValléeJean-Noël Vallée
Feb 26, 2020·Journal of Lipid Research·Zhongjie FuLois E H Smith
Feb 9, 2021·Journal of Lipid Research·Fu ZhongjieE H Smith Lois
Feb 26, 2021·Communications Biology·Warren W PanCagri G Besirli
Jul 28, 2020·Progress in Retinal and Eye Research·Robert J CassonJohn P M Wood
Apr 11, 2021·Experimental Eye Research·Zhijie WangShibo Tang
May 4, 2021·Frontiers in Molecular Neuroscience·Filipe O Viegas, Stephan C F Neuhauss
Jun 9, 2021·Annual Review of Vision Science·James B Hurley

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