M1 macrophage-derived exosomes impair beta cell insulin secretion via miR-212-5p by targeting SIRT2 and inhibiting Akt/GSK-3β/β-catenin pathway in mice.

Diabetologia
Bin QianXiao Han

Abstract

Macrophage levels are elevated in pancreatic islets, and the resulting inflammatory response is a major contributor to beta cell failure during obesity and type 2 diabetes mellitus. Previous studies by us and others have reported that exosomes released by macrophages play important roles in mediating cell-to-cell communication, and represent a class of inflammatory factors involved in the inflammatory process associated with type 2 diabetes mellitus. However, to date, no reports have demonstrated the effect of macrophage-derived exosomes on beta cells, and little is known regarding their underlying mechanisms in beta cell injury. Thus, we aimed to study the impact of macrophage-derived exosomes on islet beta cell injury in vitro and in vivo. The phenotypic profiles of islet-resident macrophages were analysed in C57BL/6J mice fed a high-fat diet (HFD). Exosomes were collected from the medium of cultured bone marrow-derived macrophages (BMDMs) and from isolated islet-resident macrophages of HFD-fed mice (HFD-Exos). The role of exosomes secreted by inflammatory M1 phenotype BMDMs (M1-Exos) and HFD-Exos on beta cell function was assessed. An miRNA microarray and quantitative real-time PCR (qPCR) were conducted to test the level of ...Continue Reading

References

Apr 21, 1999·Current Opinion in Cell Biology·Q Eastman, R Grosschedl
Mar 30, 2001·The Journal of Biological Chemistry·S FukumotoM E Lee
Jun 21, 2007·Diabetes·Jan A EhsesMarc Y Donath
Sep 4, 2009·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Enpeng ZhaoAlan D Attie
Mar 1, 2012·Nucleic Acids Research·Anaïs WanetPatricia Renard
Jun 12, 2013·Cell Metabolism·Marc Y DonathMarianne Böni-Schnetzler
Aug 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jia NieYuguang Shi
Jan 22, 2014·The Journal of Biological Chemistry·Gopalakrishnan RamakrishnanGuri Tzivion
Aug 25, 2015·Biochimica Et Biophysica Acta·Kobina EssandohGuo-Chang Fan
Apr 2, 2016·The Journal of Clinical Investigation·Paul D RobbinsCori N Booker
Jan 4, 2017·The Journal of Clinical Investigation·Kosei Eguchi, Ryozo Nagai
Jan 28, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Chunxiao WangJie Du
Apr 17, 2019·Seminars in Immunopathology·Marianne Böni-Schnetzler, Daniel T Meier
Jan 14, 2020·Journal of Cellular and Molecular Medicine·Wenjun ChenJianni Qi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adenoma, Islet Cell

Islet Cell Adenoma arises in the islet cells, which are insulin producing cells of the pancreas. These tumors can be either malignant or benign. Discover the latest research on Islet Cell Adenoma here.

Related Papers

European Review for Medical and Pharmacological Sciences
J-F LinJ-Q Zhao
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Zhi-Dong LvHai-Bo Wang
© 2021 Meta ULC. All rights reserved