M1 Macrophages Induce PD-L1 Expression in Hepatocellular Carcinoma Cells Through IL-1β Signaling

Frontiers in Immunology
Zhaoyun ZongYongyu Shi

Abstract

Hepatocellular carcinoma (HCC) is a prototype of inflammation-related cancer, harboring M1-like and M2-like tumor-associated macrophages. M1 macrophages are thought to be tumoricidal, but some studies report its pro-tumor role. The programmed cell death-ligand (PD-L) 1 expressed in HCC cells is a critical checkpoint molecule to mediate immune escape of HCC. The PD-L1 expression in HCC cells is inducible. In the present study, we ask whether M1 macrophages induce the expression of PD-L1 in HCC cells. First, an association between M1 macrophage infiltration and PD-L1 expression in HCC tissues was determined by bioinformatics and immunohistochemistry experiments. The enrichment score of M1 macrophages was correlated to PD-L1 expression in 90 HCC samples from GEO database. Besides, infiltration of CD68+HLA-DR+ M1-like macrophages correlated with PD-L1 expression level in HCC cells. Moreover, M1-conditioned media was prepared from M1 macrophages derived from THP-1 cell, RAW264.7 cell or murine bone marrow. These supernatants induced expression of PD-L1 in HCC cells. Furthermore, inflammatory cytokine IL-1β in the supernatants was identified to account for the inducible PD-L1 expression by siRNA assay and receptor blockade assay. Add...Continue Reading

References

Jan 21, 2010·Science Signaling·Axel WeberMichael Kracht
Nov 4, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rinat ZaynagetdinovTimothy S Blackwell
Aug 2, 2012·Nature Reviews. Drug Discovery·Charles A DinarelloJos W M van der Meer
Aug 29, 2012·The Journal of Experimental Medicine·Timothy O'SullivanJack D Bui
Jan 25, 2014·International Journal of Cancer. Journal International Du Cancer·Ole HelmSusanne Sebens
May 6, 2014·Cellular & Molecular Immunology·Tianxiao Wang, Jianli Wang
Feb 24, 2015·Pharmacological Reviews·Stefan SiebertIain McInnes
Sep 2, 2015·The Journal of Clinical Investigation·Lieping Chen, Xue Han
Dec 2, 2015·Advanced Drug Delivery Reviews·Yoshihiro KomoharaMotohiro Takeya
Jul 1, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Julien CalderaroJean-Michel Pawlotsky
Sep 13, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Antonio Solinas, Diego F Calvisi
May 13, 2017·Cell Reports·Angel Garcia-DiazAntoni Ribas
Jul 12, 2017·Clinical & Translational Immunology·Shalini RoyPijush K Das
Nov 17, 2017·Genome Biology·Dvir AranAtul J Butte
Jan 9, 2018·Lancet·Alejandro FornerJordi Bruix
Mar 8, 2018·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G PaselloF Calabrese
Aug 19, 2018·Cancer Immunology, Immunotherapy : CII·Yongyu Shi
Oct 6, 2018·Cell·Miguel F Sanmamed, Lieping Chen

❮ Previous
Next ❯

Citations

May 16, 2020·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Rui Caetano OliveiraMaria Augusta Cipriano
Nov 12, 2020·Cancer Immunology, Immunotherapy : CII·Ming-Fang WuHuei-Wen Chen
Mar 8, 2021·BMC Nephrology·Colleen S Curran, Jeffrey B Kopp
May 2, 2021·Biochemical and Biophysical Research Communications·Yuan HeGang Chi
May 14, 2021·Frontiers in Immunology·Katherine E LindbladAmaia Lujambio
Oct 7, 2021·Journal of Cell Communication and Signaling·Zihong WuMasoud Najafi
Nov 2, 2021·Journal of Dental Research·D WuZ Wang

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE41804
GSE121248

Methods Mentioned

BETA
biopsy
flow cytometry
PCR
electrophoresis
Immunoprecipitation
Assay
PMA
ELISA
ubiquitination

Software Mentioned

GelPro
Prism
GraphPad Prism
R studio

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here