M2 muscarinic receptors inhibit cell proliferation in human glioblastoma cell lines

Life Sciences
M FerrettiAda Maria Tata

Abstract

In the present work we investigated the expression of M2 muscarinic receptor subtype in two glioblastoma cell lines and its role in the control of cell proliferation. The M2 receptor transcript and protein expression was studied using RT-PCR and western blot analysis. (3)[H]-thymidine incorporation was used to evaluate cell proliferation in the presence or in the absence of M(2) agonist arecaidine. We demonstrated that M(2) receptor is expressed in both cell lines, although U251 cells show a higher expression level, compared to U87 cells. The activation of M(2) receptors by the agonist arecaidine decreases cell growth in a dose and time dependent manner. The anti-proliferative effect of arecaidine is also confirmed by the significant decrease of (3)[H]-thymidine incorporation in both cell lines. Moreover the M2 antagonist gallamine counteracts the arecaidine effects confirming M2 receptor involvement in glioma cell growth inhibition. These data suggest a role for M2 receptors in the inhibition of glioma cell proliferation and the possibility of exploiting these receptors as new promising tools for glioblastoma therapy.

References

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Jun 1, 1985·British Journal of Pharmacology·R B Barlow, P Weston-Smith

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Citations

Jun 3, 2015·International Immunopharmacology·Francesco AlessandriniAda Maria Tata
Oct 13, 2015·Neurochemistry International·Maria Di BariAda Maria Tata
Mar 16, 2013·Journal of Cellular and Molecular Medicine·Michela FerrettiAda Maria Tata
Dec 9, 2014·Cancer Biology & Therapy·Luca PaciniAntonella Calogero
May 21, 2016·Frontiers in Immunology·Aleli Salazar-RamiroBenjamín Pineda
Jan 1, 2013·Genes·Wymke OckengaRitva Tikkanen
Oct 9, 2019·Cells·Emily G Thompson, Harald Sontheimer
Aug 24, 2013·Oncology Reports·Miguel A Fonseca-SanchézCésar López-Camarillo
Jun 28, 2018·Biomarker Research·Kiera WalkerAnita B Hjelmeland
Mar 7, 2020·International Journal of Molecular Sciences·Ilaria CristofaroAda Maria Tata
Nov 14, 2020·International Journal of Molecular Sciences·Anna Maria LucianòAda Maria Tata
Aug 3, 2021·Experimental Cell Research·Pritam Kumar RoyMahitosh Mandal

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