PMID: 9192172Jun 1, 1997Paper

M3 muscarinic receptor mediates regulation of protein secretion in rabbit lacrimal gland

Current Eye Research
M NakamuraK Nakata

Abstract

To identify which muscarinic receptor subtypes mediate protein secretion in isolated rabbit lacrimal glands. To compare protein secretory profiles in vitro with those in rabbit tears. Rabbit lacrimal gland slices were incubated with carbachol in the presence and absence of different muscarinic antagonists. Protein secretion into the incubation medium was characterized with a Bio-Rad protein assay dye reagent in conjunction with Laemmli's method of SDS-PAGE. The medium protein profile was compared to that in tear samples. Dose dependent increases in protein secretion were elicited by carbachol between 10(-7) and 10(-4) M. During the first 20 min period, a maximal increase of 64% above the basal level was seen at the highest concentration. With 10(-4) M carbachol, the response was transient because after 100 min it decreased to its basal level. The increases in protein secretion caused by 10(-4) M carbachol were completely suppressed in the presence of 10(-5) M atropine. On the other hand, the relatively selective M1 antagonist, pirenzepine, at concentrations from 10(-6) M to 10(-4) M, had no effect on either the basal levels or the stimulatory effects of carbachol. Similarly, the relatively selective M2 antagonist, gallamine, at...Continue Reading

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Citations

Jun 27, 2012·Molecular Aspects of Medicine·Andreas EckhardHubert Löwenheim
Feb 6, 2008·Biologicals : Journal of the International Association of Biological Standardization·Vanessa ScheggSylvia M Miescher
Oct 26, 1999·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·T MurakamiK Nakata
May 15, 2010·Current Eye Research·Stina K CarlssonJ Peter Gierow
Jul 9, 2020·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·A MusayevaUNKNOWN Sicca-Preis-Jury
Dec 22, 2015·Current Molecular Biology Reports·Helen P Makarenkova, Darlene A Dartt

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