Aug 21, 2014

MacABCsm, an ABC-type tripartite efflux pump of Stenotrophomonas maltophilia involved in drug resistance, oxidative and envelope stress tolerances and biofilm formation

The Journal of Antimicrobial Chemotherapy
Yi-Tsung LinTsuey-Ching Yang

Abstract

To characterize a five gene cluster, macRS-macABCsm, in Stenotrophomonas maltophilia. The presence of macABCsm operon was verified by RT-PCR. The substrate spectrum of the MacABCsm efflux pump was investigated by mutant construction and susceptibility testing. The physiological role of MacABCsm was assessed by comparing the growth of wild-type and macABCsm mutant under different stresses. To examine the regulatory role of the two-component regulatory system (TCS) macRS in the expression of macABCsm operon, mutant construction, quantitative RT-PCR and susceptibility testing were employed. macAsm, macBsm and macCsm genes formed a three-membered operon. The MacABCsm efflux pump extruded macrolides, aminoglycosides and polymyxins and contributed to oxidative and envelope stress tolerances and biofilm formation. Inactivation of macRS TCS hardly influenced the expression of macABCsm operon and the antimicrobial susceptibility. The MacABCsm pump has physiological roles in protecting S. maltophilia from the attack of oxidative and envelope stresses and in biofilm formation, which may be the reason why it can be constitutively expressed in the absence of antibiotics and is highly conserved in S. maltophilia isolates isolated from differ...Continue Reading

Mentioned in this Paper

Aminoglycosides
Bacteriocidal Agents
Antibiotic throat preparations
Macrolide Antibiotics
Genes, Reiterated
Antifungal Antibiotics, Topical
Kinetic Polymerase Chain Reaction
Gene Deletion
Reverse Transcriptase Polymerase Chain Reaction
ATP-Binding Cassette Transporters

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