Machining protein microcrystals for structure determination by electron diffraction.

Proceedings of the National Academy of Sciences of the United States of America
Helen M E DuyvesteynDavid I Stuart

Abstract

We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration that atomic information can be retained suggests that milling could provide such detail on sections cut from vitrified cells.

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Citations

May 2, 2019·Nature Methods·Brent L Nannenga, Tamir Gonen
Oct 3, 2019·IUCrJ·Johan HattneTamir Gonen
Aug 29, 2019·Science Advances·Hongyi XuMartin Högbom
Sep 9, 2020·Nature Communications·Geoff SuttonMark Boyce
Jan 9, 2019·Frontiers in Molecular Biosciences·Brent L NannengaDan Shi
Feb 20, 2020·Structural Dynamics·Brent L Nannenga
Jul 18, 2020·F1000Research·Michael C ThompsonJose A Rodriguez
Aug 28, 2020·Frontiers in Molecular Biosciences·Emma V BealePeijun Zhang
Sep 29, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Krishna P KhakurelJakob Andreasson
Jul 28, 2019·Nature Communications·Julian T C WennmacherTim Gruene
Dec 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Michael W MartynowyczTamir Gonen
Oct 3, 2020·Structure·Michael W Martynowycz, Tamir Gonen
Mar 2, 2021·Acta Crystallographica. Section D, Structural Biology·Max T B Clabbers, Hongyi Xu
Jun 22, 2021·Annual Review of Biochemistry·Xuelang MuTamir Gonen
Aug 21, 2021·Nature Communications·Jingjing ZhaoXiaodong Zou
Sep 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Michael W MartynowyczTamir Gonen
Dec 8, 2021·Proceedings of the National Academy of Sciences of the United States of America·Michael W MartynowyczTamir Gonen

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Methods Mentioned

BETA
X-ray
electron scattering

Software Mentioned

Phenix
cppxfel
Qt5
sMX
XFEL

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