Macro to microfluidics system for biological environmental monitoring

Biosensors & Bioelectronics
Cyril DelattreChristine Peponnet

Abstract

Biological environmental monitoring (BEM) is a growing field of research which challenges both microfluidics and system automation. The aim is to develop a transportable system with analysis throughput which satisfies the requirements: (i) fully autonomous, (ii) complete protocol integration from sample collection to final analysis, (iii) detection of diluted molecules or biological species in a large real life environmental sample volume, (iv) robustness and (v) flexibility and versatility. This paper discusses all these specifications in order to define an original fluidic architecture based on three connected modules, a sampling module, a sample preparation module and a detection module. The sample preparation module highly concentrates on the pathogens present in a few mL samples of complex and unknown solutions and purifies the pathogens' nucleic acids into a few μL of a controlled buffer. To do so, a two-step concentration protocol based on magnetic beads is automated in a reusable macro-to-micro fluidic system. The detection module is a PCR based miniaturized platform using digital microfluidics, where reactions are performed in 64 nL droplets handled by electrowetting on dielectric (EWOD) actuation. The design and manuf...Continue Reading

References

Jul 12, 2003·Science·Todd H RiderMark A Hollis
Mar 10, 2004·Lab on a Chip·Phil PaikRichard B Fair
Apr 22, 2004·Lab on a Chip·M G PollackR B Fair
Sep 18, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C DelattreM A Vijayalakshmi
Nov 22, 2008·Lab on a Chip·Ramakrishna SistaVamsee Pamula
Feb 4, 2010·Lab on a Chip·Lidija MalicMaryam Tabrizian

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Citations

Jul 31, 2014·Analytical and Bioanalytical Chemistry·Maria-Nefeli TsaloglouHywel Morgan
Oct 21, 2015·Nucleic Acids Research·Tuval Ben YehezkelEhud Shapiro
Mar 31, 2015·New Biotechnology·Tadej KokaljJeroen Lammertyn
Oct 22, 2016·New Biotechnology·Elena Pérez-RuizDragana Spasic
Apr 12, 2015·Journal of Laboratory Automation·Christopher F Fronczek, Jeong-Yeol Yoon
Apr 18, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·A SchultzJ Heikenfeld
Dec 29, 2017·Environmental Science & Technology·Olivia Campana, Donald Wlodkowic

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