Macrocyclic host-dye reporter for sensitive sandwich-type fluorescent aptamer sensor

Analytical Chemistry
Cheng YangEric Peyrin

Abstract

We describe herein a novel approach for the fluorescent detection of small molecules using a sandwich-type aptamer strategy based on a signaling macrocyclic host-dye system. One split adenosine aptamer fragment was 5'-conjugated to a β-cylodextrin (CD) molecule while the other nucleic acid fragment was labeled at the 3'-end by a dansyl molecule prone to be included into the macrocycle. The presence of the small target analyte governed the assembly of the two fragments, bringing the dye molecule and its specific receptor in close proximity and promoting the inclusion interaction. Upon the inclusion complex formation, the microenvironment of dansyl was modified in such a way that the fluorescent intensity increased. Concomitantly, this supplementary interaction at the aptamer extremities induced stabilizing effects on the ternary complex. We next proposed a bivalent signaling design where the two extremities of one split aptamer fragment were conjugated to the β-CD molecule while those of the other fragment were tagged by the dansyl dye. The dual reporting dye inclusion promoted an improvement of both the signal-to-background change and the assay sensitivity. Owing to the vast diversity of responsive host-macrocycle systems avail...Continue Reading

References

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Citations

Feb 3, 2016·Analytical Chemistry·Benoit ChovelonCorinne Ravelet
Mar 26, 2016·Biosensors & Bioelectronics·Yang-Bao MiaoYijin Chen
Sep 27, 2018·Chemical Communications : Chem Comm·X ZhouJ Jayawickramarajah
Nov 5, 2020·Analytical Chemistry·Andresa B BezerraChristopher J Easley
Oct 17, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Chenze LuArnaud Buhot
Dec 7, 2018·Chemical Reviews·Teresa L MakoMindy Levine

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