PMID: 7544159Aug 3, 1995Paper

Macrocyclic lactone synthesis by lipases in water-in-oil microemulsions

Biochimica Et Biophysica Acta
G D ReesG R Stephenson

Abstract

Five microbial lipases from Chromobacterium viscosum, Candida cylindracea, Pseudomonas (source Fluka), Pseudomonas (source Genzyme) and lipoprotein lipase ex Microbial (Genzyme) have been screened for lactonisation activity towards 16-hydroxyhexadecanoic acid (HHA) in a variety of different w/o microemulsion systems. With the exception of Candida cylindracea (CC), all the lipases exhibited lactonisation activity although they were inherently more active in microemulsion systems based on the anionic surfactant sodium bis(2-ethylhexyl)sulphosuccinate (AOT) than in those based on the cationic surfactant cetyltrimethylammonium bromide (CTAB). Lactone yields are typically 50-60% and are markedly better than those reported previously using microemulsions in combination with chemical catalysts. Lipase stability is superior in the CTAB microemulsion systems, while lipase stability in the low water content AOT microemulsion systems was still good with the exception of CC lipase, which is rapidly inactivated. Buffering the water pools of AOT microemulsions using diglycine buffer at pH 8.0 improved biocatalyst stability. The lactonisation activity of lipases in CTAB w/o microemulsion systems compares favourably with that obtained using th...Continue Reading

References

Apr 5, 1990·Biotechnology and Bioengineering·D G Hayes, E Gulari

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Citations

Nov 2, 2001·Journal of Biotechnology·Mohamed GargouriMarie-Dominique Legoy
Dec 2, 2000·Biochimie·C M Carvalho, J M Cabral
Oct 21, 2016·Applied Microbiology and Biotechnology·Katarzyna CiesielskaBart Devreese
Mar 14, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Muhammad MoniruzzamanMasahiro Goto
Dec 31, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·M MoniruzzamanM Goto
Oct 11, 2003·Biotechnology Advances·H StamatisF N Kolisis

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