Macromolecular organization of the Yersinia pestis capsular F1 antigen: insights from time-of-flight mass spectrometry

Protein Science : a Publication of the Protein Society
M A TitoCarol V Robinson

Abstract

Mass spectrometry has been used to examine the subunit interactions in the capsular F1 antigen from Yersinia pestis, the causative agent of the plague. Introducing the sample using nanoflow electrospray from solution conditions in which the protein remains in its native state and applying collisional cooling to minimize the internal energy of the ions, multiple subunit interactions have been maintained. This methodology revealed assemblies of the F1 antigen that correspond in mass to both 7-mers and 14-mers, consistent with interaction of two seven-membered units. The difference between the calculated masses and those measured experimentally for these higher-order oligomers was found to increase proportionately with the size of the complex. This is consistent with a solvent-filled central cavity maintained on association of the 7-mer to the 14-mer. The charge states of the ions show that an average of one and four surface accessible basic side-chains are involved in maintaining the interactions between the 7-mer units and neighboring subunits, respectively. Taken together, these findings provide new information about the stoichiometry and packing of the subunits involved in the assembly of the capsular antigen structure. More g...Continue Reading

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Citations

Dec 31, 2002·Current Opinion in Structural Biology·Frank Sobott, Carol V Robinson
Aug 16, 2002·Molecular Microbiology·A V ZavialovS D Knight
Mar 3, 2007·Annual Review of Biochemistry·Michal Sharon, Carol V Robinson
Mar 24, 2012·Journal of Medical Microbiology·E D Williamson, P C F Oyston
Oct 3, 2006·Journal of Microbiological Methods·Martin H F MeyerMichael Keusgen
Apr 2, 2005·Antonie van Leeuwenhoek·Petra C F Oyston, Karen E Isherwood
Mar 14, 2013·Clinical and Experimental Immunology·E D Williamson, P C F Oyston
Aug 28, 2013·Expert Review of Anti-infective Therapy·Petra C F Oyston, E Diane Williamson
Aug 10, 2002·Molecular & Cellular Proteomics : MCP·Kenneth E Neet, J Ching Lee
Mar 20, 2003·Journal of Mass Spectrometry : JMS·Martijn W H PinkseAlbert J R Heck
Jan 18, 2003·Journal of Pharmaceutical Sciences·Duane T BrandauC Russell Middaugh

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