PMID: 7544073Apr 1, 1995Paper

Macromolecular permeability of chick wing microvessels: an intravital confocal study

Anatomy and Embryology
R N Feinberg, E Cafasso

Abstract

The development of the vertebrate limb requires the formation of a normal vasculature to nurture the soft and hard tissue phenotypes. The pattern of embryonic limb bud vessels has been extensively studied, but little is known about the permeability characteristics of the developing circulation. In the present study, the microvascular endothelial cell phenotype was examined by in vivo confocal microscopy following the systemic injection of a graded series of fluorescent dextrans (40,000, 70,000, 150,000 molecular weight) into chick embryos at stages 21-23 in order to determine how selective is the endothelial lining of microvessels as a partition between the blood vessels and the interstitium. Videodensitometry, over a gray scale range of 0-255, was used to quantitate the amount of tracer found within the interstitial compartment of the limb. The tracers of larger molecular weight (70,000, 150,000) were confined exclusively to the vascular lumina, whereas that of smaller molecular weight (40,000) was found to cause perivascular brightening due to extravasation into the surrounding interstitium. The reported differences in permeability were not dependent upon the stage of the embryo used in this study, but were due to the size of...Continue Reading

References

Jan 1, 1975·Microvascular Research·Y Nakamura, H Wayland
Dec 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·V Hamburger, H L Hamilton
May 1, 1991·Microvascular Research·J J Tiedeken, C M Rovainen
Sep 1, 1991·The Anatomical Record·R N Feinberg, D M Noden
Jan 1, 1991·Anatomy and Embryology·R N FeinbergL Sweetman
Mar 1, 1988·Developmental Biology·R F Drushel, A I Caplan
Dec 1, 1974·Developmental Biology·R AuerbachJ Folkman
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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Citations

Apr 1, 1996·Die Naturwissenschaften·J Wilting, B Christ

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