PMID: 26786768Jan 21, 2016Paper

Macromolecular Powder Diffraction: Ready for genuine biological problems

Protein and Peptide Letters
Fotini Karavassili, Irene Margiolaki

Abstract

Knowledge of 3D structures of biological molecules plays a major role in both understanding important processes of life and developing pharmaceuticals. Among several methods available for structure determination, macromolecular X-ray powder diffraction (XRPD) has transformed over the past decade from an impossible dream to a respectable method. XRPD can be employed in biosciences for various purposes such as observing phase transitions, characterizing bulk pharmaceuticals, determining structures via the molecular replacement method, detecting ligands in protein-ligand complexes, as well as combining micro-sized single crystal crystallographic data and powder diffraction data. Studies using synchrotron and laboratory sources in some standard configuration setups are reported in this review, including their respective advantages and disadvantages. Methods presented here provide an alternative, complementary set of tools to resolve structural problems. A variety of already existing software packages for powder diffraction data processing and analysis, some of which have been adapted to large unit cell studies, are briefly described. This review aims to provide necessary elements of theory and current methods, along with practical ...Continue Reading

Citations

Apr 8, 2020·Acta Crystallographica. Section D, Structural Biology·Fotini KaravassiliI Margiolaki
Aug 25, 2020·Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials·Stavroula FiliIrene Margiolaki
Dec 6, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Stavroula FiliIrene Margiolaki

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