Macrophage CD163 expression in cerebrospinal fluid: association with subarachnoid hemorrhage outcome

Journal of Neurosurgery
Ajith J ThomasKhalid A Hanafy

Abstract

Even though heme-induced cerebral inflammation contributes to many of the adverse sequelae seen in patients with subarachnoid hemorrhage (SAH), little is known about the mechanism; mouse models have shown a critical role for macrophages/microglia. Macrophage CD163 is a hemoglobin scavenger receptor involved in blood clearance after SAH. The authors hypothesized that the modified Fisher score is independently associated with cerebrospinal fluid (CSF) macrophage CD163 expression on postictal day 1, and that CSF macrophage CD163 expression is associated with 1-month neurological outcome. CSF macrophages from 21 SAH and 28 unruptured aneurysm patients (control) were analyzed for CD163 expression using flow cytometry and confocal microscopy on postictal day 1. Significant associations with modified Fisher scale grades or modified Rankin Scale scores were determined using linear regression and a matched case control analysis. CSF macrophage CD163 expression was significantly increased in SAH patients compared with controls (p < 0.001). The modified Fisher scale (mF) grades (β = 0.407, p = 0.005) and CSF bilirubin concentrations (β = 0.311, p = 0.015) were positively and independently associated with CSF macrophage CD163 expression wh...Continue Reading

References

Jun 1, 1997·Journal of Leukocyte Biology·P Antal-SzalmasK P Van Kessel
Feb 24, 2001·Nature·M KristiansenS K Moestrup
Nov 21, 2007·Circulation·Tomohiro AokiNobuo Hashimoto
Oct 15, 2008·Neuroscience Letters·Ian GaleaTracey A Newman
Dec 10, 2008·Stroke; a Journal of Cerebral Circulation·Julian Cahill, John H Zhang
Nov 26, 2009·Anesthesiology·Glynne D Stanley
Mar 3, 2012·Journal of Neurochemistry·James GaleaIan Galea
Apr 22, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Abu OsmanShinya Suzu
Jul 17, 2014·Critical Care : the Official Journal of the Critical Care Forum·Ségolène MrozekThomas Geeraerts
Apr 9, 2015·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Elizabeth NewellRobert S B Clark
May 2, 2015·Stroke; a Journal of Cerebral Circulation·Kenji ShimadaTomoki Hashimoto
May 27, 2015·The Journal of Clinical Investigation·Nils SchallnerKhalid A Hanafy
Sep 26, 2015·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·BaoHua LiuXiaoTong Wang
Oct 1, 2015·Neurosurgery·Zahrah TaufiqueJ Michael Schmidt
Jan 16, 2016·Stroke; a Journal of Cerebral Circulation·Patrick GarlandIan Galea
Apr 30, 2016·Stroke; a Journal of Cerebral Circulation·Shenglong CaoGuohua Xi
Jul 2, 2016·Journal of Neurochemistry·Jing Chen-Roetling, Raymond F Regan
Mar 31, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jenna L LeclercSylvain Doré
Apr 1, 2017·Stroke; a Journal of Cerebral Circulation·Ran LiuGuohua Xi
Apr 11, 2017·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Andrew L A GartonE Sander Connolly
Jul 28, 2017·Stroke; a Journal of Cerebral Circulation·Nils SchallnerLeo E Otterbein

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Citations

Aug 23, 2019·CNS Neuroscience & Therapeutics·Yingfeng WanGuohua Xi
Aug 29, 2019·Current Neurology and Neuroscience Reports·Katharina M Busl
Jun 24, 2020·Nature Communications·Jinman ChenYongjun Wang
Sep 30, 2020·Translational Stroke Research·Fenghui YeGuohua Xi
Nov 20, 2020·Redox Biology·Cristina Fernández-MendívilManuela G López
Jan 28, 2021·International Journal of Molecular Sciences·Ayon BhattacharyaSylvain Doré
Jun 24, 2021·Journal of Neurochemistry·Jiru ZhouHua Feng
Jul 21, 2021·Stroke; a Journal of Cerebral Circulation·Anan ShtayaAtticus H Hainsworth

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Methods Mentioned

BETA
flow cytometry
FACS
confocal microscopy
biopsies

Software Mentioned

FlowJo
SPSS

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