PMID: 7539144May 23, 1995Paper

Macrophage-colony-stimulating factor regulates expression of the integrins alpha 4 beta 1 and alpha 5 beta 1 by murine bone marrow macrophages

Proceedings of the National Academy of Sciences of the United States of America
M ShimaF P Ross

Abstract

We observed that when monocyte/macrophage precursors derived from murine bone marrow were treated with macrophage-colony-stimulating factor (M-CSF), there was a dose-dependent increase in both the number of adherent cells and the degree to which the cells were highly spread. Attachment was supported by fibronectin, but not by vitronectin or laminin, suggesting that the integrins alpha 4 beta 1 and/or alpha 5 beta 1 might mediate this event. Binding to fibronectin was blocked partially by antibodies to either integrin, and inhibition was almost complete when the antibodies were used in combination. By a combination of surface labeling with 125I and metabolic labeling with [35S]methionine and [35S]cysteine, we demonstrated that M-CSF treatment led to increased synthesis and surface expression of the two beta 1 integrins. Since attachment to fibronectin and/or stromal cells plays an important role in the maturation of other hematopoietic lineages, we propose that the action of M-CSF in the differentiation of immature monocytes/macrophages includes stimulated expression of the integrins alpha 4 beta 1 and alpha 5 beta 1, leading to interactions with components of the marrow microenvironment necessary for cell maturation.

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Citations

Jan 1, 1996·Immunologic Research·J Roman
Nov 15, 2002·International Journal of Hematology·Jean E Sanders
May 7, 2011·Expert Reviews in Molecular Medicine·Susan R Rittling
Feb 16, 2002·Journal of Hematotherapy & Stem Cell Research·Alexander Schmeisser, Ruth H Strasser
Nov 19, 2010·Journal of Leukocyte Biology·Michelle Y AbshireAmy H Bouton
Sep 9, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Beatriz CesarDorly de Freitas Buchi
Mar 24, 2007·Micron : the International Research and Review Journal for Microscopy·Beatriz CesarDorly de Freitas Buchi
Jul 14, 2017·American Journal of Physiology. Cell Physiology·Hyo-Jung ChooGrace K Pavlath
Jul 9, 2004·Scandinavian Journal of Immunology·S B GeutskensW Savino
Aug 1, 2018·Stem Cells·Kristina ProbstBent Brachvogel
Nov 2, 2004·European Journal of Immunology·Sacha Brigitte GeutskensWilson Savino

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