Macrophage Cytokines Enhance Cell Proliferation of Normal Prostate Epithelial Cells through Activation of ERK and Akt

Scientific Reports
Tu Dang, Geou-Yarh Liou

Abstract

Macrophage infiltrations (inflammation) are associated with prostate disorders such as prostatitis, prostatic hyperplasia and prostate cancer. All prostate disorders have elevated cell proliferation, and are initiated from normal prostate epithelial cells. To date, the mechanism of how macrophages regulate normal prostate epithelial cell proliferation remains largely unknown. Using a 3D co-culture system, we here show that Raw 264.7 macrophages increased cell proliferation of normal prostate epithelial PZ-HPV-7 cells. In addition, these Raw 264.7 macrophages expressed higher levels of Ym1 and CD206. We further identify macrophage-secreted cytokines including CCL3, IL-1ra, osteopontin, M-CSF1 and GDNF as mediators for potentiating PZ-HPV-7 cell proliferation in 3D. All these cytokines differentially activated ERK and Akt. Blockade of both kinases through their inhibitors hindered macrophage-induced cell proliferation of PZ-HPV-7 cells. Hence, our data provide mechanistic insight of how inflammation may contribute to development of prostatic diseases at a very early stage through augment of cell proliferation of normal prostate epithelial cells.

References

Jan 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·A P GadeauC Desgranges
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
Jan 11, 2003·Circulation Research·Mimi Tamamori-AdachiMasa-Aki Ikeda
Aug 15, 2003·Molecular and Cellular Biology·Aurelian RaduMaria-Magdalena Georgescu
Oct 4, 2003·Clinical & Experimental Metastasis·Gabriele CarlinfanteGöran Andersson
Jan 10, 2004·The Journal of Urology·Elizabeth A Platz, Angelo M De Marzo
Nov 9, 2004·Trends in Immunology·Alberto MantovaniMassimo Locati
Dec 16, 2004·The Journal of Clinical Investigation·Shigetada FurukawaIichiro Shimomura
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tatsuma OkazakiHidetada Sasaki
Dec 7, 2005·International Journal of Cancer. Journal International Du Cancer·Shiva S ForootanYouqiang Ke
Jan 21, 2006·Cancer Research·Ani C KhodavirdiPradip Roy-Burman
Dec 1, 2007·Cancer Metastasis Reviews·Philip Y Wai, Paul C Kuo
Jan 5, 2008·The Journal of Biological Chemistry·Jeong-Ah KangAmittha Wickrema
Mar 23, 2010·Cell·Sergei I GrivennikovMichael Karin
Apr 8, 2010·Free Radical Research·Geou-Yarh Liou, Peter Storz
Nov 27, 2010·Journal of Hepatology·Guillaume VialXavier M Leverve
Jan 12, 2011·Annual Review of Immunology·Margaret F Gregor, Gökhan S Hotamisligil
Jun 21, 2011·International Journal of Molecular Sciences·Alba Fernández-SánchezJosé A Morales-González
Jan 4, 2012·Histopathology·Karen S Sfanos, Angelo M De Marzo
Mar 2, 2012·The Journal of Clinical Investigation·Antonio Sica, Alberto Mantovani
Mar 31, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Oleksandr N KryvenkoBenjamin A Rybicki
Apr 24, 2012·Journal of Nutrition and Metabolism·Faloia EmanuelaBoscaro Marco
Jul 21, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Marsha L QuickPraveen Thumbikat
Oct 26, 2012·The Journal of Pathology·Alberto MantovaniMassimo Locati
Feb 23, 2013·Critical Reviews in Immunology·Charles D Mills

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Citations

Apr 20, 2019·Scandinavian Journal of Immunology·Caiyun QianDaya Luo
May 2, 2020·The Prostate·Petra PopovicsMagdalena M Grabowska
Mar 18, 2020·Cell Biology International·Nayara F C CastroPatrícia S L Vilamaior
Aug 6, 2020·Clinical, Cosmetic and Investigational Dermatology·Dongsoo KimJunghwa Kang
Nov 30, 2018·Frontiers in Immunology·Jürgen PollheimerMartin Knöfler
Feb 19, 2020·Molecular Genetics & Genomic Medicine·Abolfazl Zendehdel, Maryam Roham
Sep 20, 2020·International Journal of Molecular Sciences·Fabrizio FontanaPatrizia Limonta
Jan 13, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bo-Ram JinHyo-Jin An
Jan 30, 2021·Cytokine·Nataša Todorović-Raković
Dec 17, 2020·Biomedicines·Yu-Ching FanYuan-Chin Tsai
May 3, 2021·Molecular and Cellular Endocrinology·Yi-Yi WangRan Xu
May 31, 2021·Cancer Letters·Jack FreelandAndrew S Goldstein
Jun 15, 2021·The Prostate·Nayara Fernanda da Costa CastroPatrícia Simone Leite Vilamaior
Jul 22, 2021·Cell Biology International·Ellen Cristina Rivas LeonelSebastião Roberto Taboga
Aug 20, 2021·Scientific Reports·Menka KhoobchandaniKattesh V Katti

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Methods Mentioned

BETA
profiler
PCR
X-ray

Software Mentioned

Prism
GraphPad

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