Macrophage foam cell formation is augmented in serum from patients with diabetic angiopathy

Diabetes Research and Clinical Practice
Xinglong CuiTomoichiro Asano

Abstract

The differentiation of macrophages into cytokine-secreting foam cells plays a critical role in the development of diabetic angiopathy. J774.1, a murine macrophage cell line, reportedly differentiates into foam cells when incubated with oxidized LDL, ApoE-rich VLDL or WHHLMI (myocardial infarction-prone Watanabe heritable hyperlipidemic) rabbit serum. In this study, serum samples from Type 2 diabetic patients were added to the medium with J774.1 cells and the degree of foam cell induction was quantified by measuring lipid accumulation. These values were calculated relative to the activities of normal and WHHLMI rabbit sera as 0% and 100%, respectively, and termed the MMI (Macrophage Maturation Index). These MMI values reflected intracellular lipids, including cholesteryl ester assayed by GC/MS. Statistical analysis revealed MMI to correlate positively and independently with serum triglycerides, the state of diabetic retinopathy, nephropathy and obesity, but negatively with administration of alpha-glucosidase inhibitors or thiazolidinediones. Taken together, our results suggest that this novel assay may be applicable to the identification of patients at risk for rapidly progressive angiopathic disorders.

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Citations

Sep 3, 2016·Multiple Sclerosis : Clinical and Laboratory Research·Shreya MukhopadhyayMurali Ramanathan
Nov 19, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Akifumi KushiyamaTomoichiro Asano
May 31, 2020·International Journal of Molecular Sciences·Sandra Rayego-MateosJesús Egido
Oct 23, 2019·Frontiers in Cellular and Infection Microbiology·Cristian Alfredo Segura-CerdaMario Alberto Flores-Valdez
Apr 16, 2020·International Journal of Molecular Sciences·Lucas Opazo-RíosJesús Egido

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