Macrophage-Mediated Effects of Airborne Fine Particulate Matter (PM2.5 ) on Hepatocyte Insulin Resistance in Vitro

ACS Omega
Ran LiTong Zhu

Abstract

Fine particulate matter (PM2.5) pollution poses significant health risks worldwide, including metabolic syndrome-related diseases with the characteristic feature of insulin resistance. However, the mechanism and influencing factors of this effect are poorly understood. In this serial in vitro study, we aimed at testing the hypothesis that macrophage-mediated effects of PM2.5 on hepatic insulin resistance depend on its chemical composition. Mouse macrophages were exposed to PM2.5 that had been collected during summer or winter in Beijing, which represented different compositions of PM2.5. Thereafter, hepatocytes were treated with macrophage-conditioned medium (CM). PM2.5 induced interleukin-6, tumor necrosis factor-α, and monocyte chemoattractant protein-1 expression and secretion in macrophages, particularly after winter PM2.5 exposure. Correspondingly, winter CM weakened hepatocellular insulin-stimulated glucose consumption. Further investigation revealed that the normal insulin pathway was suppressed in winter CM-treated hepatocytes, with increased phosphorylation of insulin receptor substrate 1 at serine residue 307 (Ser307) and decreased phosphorylation of protein kinase B (PKB/AKT) and forkhead box transcription factor O1 ...Continue Reading

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Citations

May 28, 2020·Science China. Life Sciences·Xiaoyu WangChunying Chen
Jan 21, 2021·Experimental Biology and Medicine·Lisa Kobos, Jonathan Shannahan
Feb 11, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Paige FletcherAndrij Holian
Feb 22, 2021·The Science of the Total Environment·Qisong XingNan Sang
Dec 7, 2018·Environmental Science & Technology·Courtney RoperRobert L Tanguay

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Methods Mentioned

BETA
environmental stress
PCR
electrophoresis
reverse transcription PCR

Software Mentioned

Image J
FCAP Array

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