Abstract
Fibroblast proliferation is a common manifestation of chronic inflammatory diseases, including rheumatoid arthritis (RA), Crohn's disease and ulcerative colitis, etc. To alleviate patient suffering, the mechanism underlying fibroblast proliferation should be elucidated. CCK-8 assay was used to assess the stimulatory effect of LPS and macrophage migration inhibitory factor (MIF) on fibroblast proliferation. Then, TLR4 expression on fibroblast cell membrane was carried out by confocal scanning microscopy. Finally, real-time fluorescent quantitative PCR and flow cytometry were applied to determine the expression of TLR4 after MIF challenge. LPS alone directly stimulated the fibroblast proliferation. In addition, MIF showed co-stimulatory effect on LPS-induced fibroblast proliferation. Interestingly, fibroblast overtly expressed TLR4 without stimulation. After MIF stimulation, real-time PCR showed TLR4 mRNA levels were increased by about 33% in the fibroblasts; in agreement, TLR4 expression on the fibroblast membrane was increased by about 20%, as shown by flow cytometry. These findings indicated MIF elevates TLR4 expression in fibroblast, enhancing LPS-induced cell proliferation.
References
Jan 1, 1991·Microbiology and Immunology·S HiroseT Osawa
Aug 1, 1991·Lymphokine and Cytokine Research·S OkiT Osawa
Jul 1, 1966·Science·B R Bloom, B Bennett
Jun 1, 1994·The Journal of Experimental Medicine·T CalandraR Bucala
Sep 1, 1996·British Journal of Rheumatology·K TeratoM M Griffiths
Dec 6, 1997·Biochemical and Biophysical Research Communications·D Chakravortty, K S Kumar
Aug 7, 1998·Journal of Endodontics·V LertchirakarnH H Messer
Dec 8, 1998·Journal of Periodontal Research·A TakemuraS Hamada
Oct 19, 1999·Microbiology and Immunology·K TakahashiT Yokochi
Apr 1, 2000·British Journal of Pharmacology·S Yoshino, M Ohsawa
May 3, 2000·Immunology·S YoshinoM Ohsawa
Jan 10, 2002·Nature·T RogerT Calandra
Jan 25, 2002·Phytotherapy Research : PTR·H YangG W Cherry
Sep 25, 2009·Cytokine·Xinyan LiRichard I Tapping
Dec 2, 2009·Cell Biology International·Cai Xiao-junZhang Mei
Dec 19, 2009·Respiratory Research·ZhengYu HeHong Jiang
Aug 11, 2010·Inflammation·Eun-Gyeong LeeWan-Hee Yoo
May 9, 2012·PloS One·Zhengyu HeXiangrui Wang
Nov 20, 2013·Seminars in Immunology·Diana Boraschi, Aldo Tagliabue
Jan 7, 2014·Cell & Bioscience·Zhengyu HeXiangrui Wang
Oct 10, 2014·Biological Research·Won-Seok LeeWan-Hee Yoo
Mar 31, 2015·Mediators of Inflammation·Yung-Chun ChuangTrai-Ming Yeh
Citations
Feb 9, 2018·Digestion·Zhiquan FuRenye Que
Aug 30, 2017·Cell Biology International·Haibing LanFang Yao
Feb 14, 2019·Pediatric Research·Shahana PerveenMohamed Ahmed
Jun 23, 2019·International Journal of Biometeorology·Laura María Melgar-SánchezInés Martínez-Galán
May 3, 2018·International Journal of Molecular Medicine·Marianna De ColliSusi Zara
Jul 13, 2018·Frontiers in Immunology·César TrifoneGabriela Turk
Aug 21, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Juan CaiHui Liu