Macrophage migration inhibitory factor has a MHC class I-like motif and function

Scandinavian Journal of Immunology
D J GibbingsA D Befus

Abstract

Macrophage migration inhibitory factor (MIF) is found in immune-privileged sites and inhibits cytotoxicity mediated by CD3-ve lymphokine-activated killer cells (LAK). The mechanism by which MIF attenuates LAK cytotoxicity is unknown. We provide evidence that MIF has a major histocompatibility complex (MHC) class I-like motif. A monoclonal antibody (OX18) that binds a conserved region of rat MHC class I proteins binds native MIF. Anti-MIF polyclonal antibodies bind MHC class I. Epitope mapping suggests OX18 binds a loop of MHC class I bound by several receptors for MHC class I. A sequence (PRPEG) within the proposed OX18-binding site on MHC class I exists with a short insertion in MIF. OX18 does not bind MIF that is denatured by SDS-PAGE. This suggests the OX18 epitope is dependent on higher order structure in MIF. Interestingly, MIF inhibits binding of tetramers of MHC class I (H2D(b)) to LAK cells, suggesting it may bind to receptors for MHC class I. MIF may be an example where small regions of MHC class I are used by endogenous and viral proteins to control cytotoxicity mediated by immune cells.

References

Sep 1, 1992·European Journal of Immunology·V CerundoloA Townsend
Jun 1, 1992·The Journal of Experimental Medicine·S C JamesonJ C Howard
Nov 25, 1988·Nucleic Acids Research·F Corpet
Nov 6, 1998·The Journal of Experimental Medicine·H ChenH Metzger
Jan 20, 1999·The Journal of Experimental Medicine·H KubagawaM D Cooper
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A C ReppJ Y Niederkorn
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R TrottaB Perussia
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R AbeC N Metz
May 30, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Robert KleemannJürgen Bernhagen
Jun 5, 2003·The Journal of Experimental Medicine·Lin LengRichard Bucala
Jul 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mitsunori ShiroishiKatsumi Maenaka
Sep 23, 2003·Nature Reviews. Immunology·Thierry Calandra, Thierry Roger

❮ Previous
Next ❯

Related Concepts

Related Feeds