Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation

PloS One
Bong-Sung KimN Pallua

Abstract

Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine and has been implicated in inflammatory diseases. However, little is known about the regulation of MIF in adipose tissue and its impact on wound healing. The aim of this study was to investigate MIF expression in inflamed adipose and determine its role in inflammatory cell recruitment and wound healing. Adipose tissue was harvested from subcutaneous adipose tissue layers of 24 healthy subjects and from adipose tissue adjacent to acutely inflamed wounds of 21 patients undergoing wound debridement. MIF protein and mRNA expression were measured by ELISA and RT-PCR. Cell-specific MIF expression was visualized by immunohistochemistry. The functional role of MIF in cell recruitment was investigated by a chemotaxis assay and by flow cytometry of labeled macrophages that were injected into Mif-/-and wildtype mice. Wound healing was evaluated by an in vitro scratch assay on human fibroblast monolayers. MIF protein levels of native adipose tissue and supernatants from acutely inflamed wounds were significantly elevated when compared to healthy controls. MIF mRNA expression was increased in acutely inflamed adipose tissue indicating the activation of MIF gene transcrip...Continue Reading

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Citations

Mar 16, 2016·Scientific Reports·Milana KokosarElisabet Stener-Victorin
Jan 17, 2017·Plastic and Reconstructive Surgery·Yi YuanFeng Lu
Apr 26, 2017·International Journal of Obesity : Journal of the International Association for the Study of Obesity·H-J KimB Cho
Apr 11, 2019·Journal of Cellular and Molecular Medicine·Jin H LiHui Yao Lan
Jan 22, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bong-Sung KimRichard Bucala
Apr 14, 2020·Journal of Plastic Surgery and Hand Surgery·Bong-Sung KimGerrit Grieb
Aug 22, 2020·Tissue Engineering. Part B, Reviews·Xihang ChenYi Yuan
Sep 9, 2016·Journal of Cellular and Molecular Medicine·Bong-Sung KimRichard Bucala
Aug 10, 2021·Frontiers in Physiology·Elena HofmannBong-Sung Kim

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Methods Mentioned

BETA
Protein Assay
ELISA
density-gradient centrifugation
FCS
lavage
flow cytometry

Software Mentioned

ImageJ
FlowJo
Photoshop
GraphPad Prism

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