Macrophage migration inhibitory factor is increased in the urine of patients with urinary tract infection: macrophage migration inhibitory factor-protein complexes in human urine

The Journal of Urology
K Meyer-SieglerPedro L Vera

Abstract

MIF is a proinflammatory cytokine present in preformed stores in human urothelium. In animal models of bladder inflammation, including bacterial cystitis, MIF is up-regulated in the bladder and released from the bladder as a high molecular weight complex. We compared urine MIF amounts in patients with UTI to that in patients without UTI, and we examined and identified MIF-protein complexes in urine. Using enzyme-linked immunosorbent assay we compared MIF levels in the urine of 14 patients with UTI to levels in 16 controls with no UTI. Western blotting under native, denaturing and reducing conditions was done to examine MIF complexes found in urine. Mass spectrometry identified MIF associated proteins in urine, while co-immunoprecipitation confirmed the associations. Mean urine MIF amounts +/- SEM determined by enzyme-linked immunosorbent assay were significantly greater in 14 patients with UTI compared to that in 16 controls (1.96 +/- 0.40 vs 0.59 +/- 0.09 ng/mg creatinine, p <0.01). Western blotting under denaturing conditions showed several high molecular weight complexes (100 to 165 kDa) that increased in UTI urine as well as typical, monomeric MIF (12 kDa). Mass spectrometry identified associated MIF proteins, including cer...Continue Reading

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Citations

Sep 19, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Hasan OtukeshSaeed Mahdavi
Oct 21, 2009·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Esra SevketogluSami Hatipoglu
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Jul 12, 2013·PloS One·Valeriya R SamyginaDmitri I Svergun
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May 12, 2007·Journal of the American Geriatrics Society·Sharon K InouyeGeorge A Kuchel
Jan 3, 2007·Journal of the American Geriatrics Society·John A Taylor, George A Kuchel
Feb 4, 2010·Neurourology and Urodynamics·Pedro L VeraKatherine L Meyer-Siegler
Jun 21, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Valeria A KostevichVadim B Vasilyev
Aug 24, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Shin Young LeeMoo Yeol Lee
May 29, 2015·PloS One·Dimitrios E KouzoukasPedro L Vera
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Aug 15, 2019·International Urogynecology Journal·Thais F MagalhaesJorge M Haddad

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