Abstract
To evaluate the phenotype of macrophages in the cornea and conjunctiva of C57BL/6 mice with induced experimental dry eye. C57BL/6 mice exposed to desiccating stress (DS) were evaluated at 1, 5, and 10 days and C57BL/6 mice maintained in non-stressed environment were used as controls. Whole eyes and adnexa were excised for histology or used for gene expression analysis. Location and phenotype of macrophages infiltrating the cornea and conjunctiva was evaluated by immunofluorescence analysis. Quantitative polymerase chain reaction evaluated macrophage markers and T cell-related and inflammatory cytokine expression in cornea and conjunctiva. Immunofluorescence staining demonstrated that macrophages reside in the conjunctiva of control and dry eye mice and their number did not change with DS. Real-time RT-PCR demonstrated that the level of M1 macrophage marker, iNOS, increased prominently in the conjunctiva at DS 10 days. In contrast, there was a non-significant decrease of the M2 marker Arg1 with DS. The levels of inflammatory cytokine, IL-12a mRNA transcript in the conjunctiva increased significantly at DS1 and decreased at DS5, while levels of IL-18 were significantly increased at DS 10. Macrophages reside in the ocular surface ...Continue Reading
References
Sep 17, 1999·Current Eye Research·S C PflugfelderD Monroy
Oct 29, 2002·Trends in Immunology·Alberto MantovaniAntonio Sica
Jan 30, 2003·Journal of Leukocyte Biology·David M Mosser
Nov 9, 2004·Trends in Immunology·Alberto MantovaniMassimo Locati
Nov 24, 2004·Investigative Ophthalmology & Visual Science·Lihui LuoStephen C Pflugfelder
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert D StoutJill Suttles
Dec 3, 2005·Nature Reviews. Immunology·Siamon Gordon, Philip R Taylor
Nov 1, 2006·The Ocular Surface·Stephen C Pflugfelder
May 26, 2007·Investigative Ophthalmology & Visual Science·Cintia S De PaivaStephen C Pflugfelder
Dec 22, 2007·International Immunology·Chi-Shiun ChiangWilliam H McBride
Nov 26, 2008·Nature Reviews. Immunology·David M Mosser, Justin P Edwards
Feb 27, 2010·Diabetes·Merav E ShaulMartin S Obin
Sep 22, 2010·Nature Immunology·Subhra K Biswas, Alberto Mantovani
Sep 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chris S SchaumburgMichael E Stern
Oct 21, 2011·Nature Immunology·Stephen J GalliThomas A Wynn
Jan 11, 2012·Archives of Ophthalmology·William StevensonReza Dana
Mar 2, 2012·The Journal of Clinical Investigation·Antonio Sica, Alberto Mantovani
Jul 10, 2012·The American Journal of Pathology·Delu ZhouNancy A McNamara
Sep 26, 2012·The Journal of Pathology·Ravid Shechter, Michal Schwartz
Oct 26, 2013·Investigative Ophthalmology & Visual Science·Joo Youn OhWon Ryang Wee
Nov 14, 2013·PloS One·Terry G CourseyCintia S de Paiva
Jan 11, 2014·Experimental & Molecular Medicine·Dong-Im ChoYoungkeun Ahn
Citations
Jul 15, 2015·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Laura SchöllhornClaus Cursiefen
Aug 5, 2016·Scientific Reports·Yu YangLin Lu
Mar 11, 2017·International Ophthalmology Clinics·Cintia S de Paiva
Jul 17, 2018·International Immunology·Yangyan XiaoStephen C Pflugfelder
Aug 2, 2019·Investigative Ophthalmology & Visual Science·Neal E MecumIan D Meng
Aug 31, 2018·Mediators of Inflammation·José L ReyesAlicia Vázquez Mendoza
Feb 25, 2017·Pflügers Archiv : European journal of physiology·Holly R ChinnerySamantha J Dando
Apr 2, 2020·Investigative Ophthalmology & Visual Science·Huan HeZhirong Lin
Feb 21, 2021·Stem Cells and Development·Jan KosslVladimir Holan
Feb 24, 2021·The Ocular Surface·Jeremias G Galletti, Cintia S de Paiva
Apr 17, 2021·The Ocular Surface·Archana Padmanabhan NairSwaminathan Sethu
Aug 8, 2021·International Journal of Molecular Sciences·Sang Min LeeVinay Kumar Aakalu
Aug 28, 2021·International Journal of Molecular Sciences·Tsung-Chuan HoYeou-Ping Tsao
Oct 11, 2020·Experimental Eye Research·Stephen C Pflugfelder, Michael E Stern