Macrophage targeting with technetium-99m labelled J001 acylated poly-galactoside for scintigraphy of inflammation: optimization and assessment of imaging specificity in experimental arthritis

European Journal of Nuclear Medicine
E Miot-NoiraultA Le Pape

Abstract

J001, an acylated poly-(1,3)-galactoside purified from the membrane of Klebsiella pneumoniae, associates selectively with macrophages via the binding to CD11b and CD14 molecules. Inflammatory foci known to recruit macrophages could thus be imaged with technetium-99m labelled J001. This study aims to define the optimal scintigraphic protocol for 99mTc-J001 imaging and to evaluate the specificity of J001 scans. A dose range study was conducted in rabbits with immunological arthritis using six different specific activities ranging from 370 to 11840 MBq·mg-1 while the intravenously injected activity was constant (37 MBq) Radiochemical purity for each preparation was documented together with the in vivo stability of the 99mTc-J001 complex using exclusion-diffusion radioHPLC of serum collected 1 h after radiopharmaceutical administration. Scintigraphic images were recorded at 2, 3 and 4h and analysed using indexes calculated from regions of interest. Specificity of the macrophage imaging was assessed by comparison with scans obtained after administration of 99mTcO4(- )or 99mTc-albumin nanocolloids. A protocol of plasma transfusion was also used to inject 99mTc-J001 after complete removal of radioactive colloids likely to be ge...Continue Reading

References

Jul 1, 1992·Scandinavian Journal of Immunology·Z HmamaJ P Revillard
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Citations

Sep 2, 1998·European Journal of Drug Metabolism and Pharmacokinetics·A D VarvarigouP Kostamis
Aug 8, 2014·Arthritis Research & Therapy·Stéphanie PutPatrick Matthys
Jun 1, 1996·European Journal of Nuclear Medicine·W J OyenF H Corstens
Jun 29, 2010·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Cláudia Borges BrasileiroTarcísio Passos Ribeiro de Campos

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