Macrophage tolerance response to Aggregatibacter actinomycetemcomitans lipopolysaccharide induces differential regulation of tumor necrosis factor-alpha, interleukin-1 beta and matrix metalloproteinase 9 secretion

Journal of Periodontal Research
S-I Tanabe, D Grenier

Abstract

The lipopolysaccharide of Aggregatibacter actinomycetemcomitans, a potent stimulator of the immune system, induces the secretion of inflammatory mediators that modulate periodontal tissue destruction. In this study, we investigated the tolerance response of human macrophages to stimulation with A. actinomycetemcomitans lipopolysaccharide. U937 monocytes were differentiated into adherent macrophage-like cells by treatment with phorbol myristic acid. Macrophage-like cells were then pretreated for 24 h with either 0.01 or 0.1 microg/mL LPS A. actinomycetemcomitans. Culture medium supernatants were removed and cells were restimulated with LPS at 1 microg/mL. Cell-free supernatants were collected after 24 h of stimulation and analyzed by ELISA for TNF-alpha, IL-1 beta, IL-6, IL-8, PGE(2) and MMP-9. Phorbol myristic acid-differentiated U937 macrophages treated with low doses of lipopolysaccharide developed tolerance to subsequent lipopolysaccharide treatments, resulting in significantly reduced secretion of tumor necrosis factor-alpha. However, this tolerance response was associated with increased secretion of interleukin-1 beta and matrix metalloproteinase 9, whereas the secretion of interleukin-6, interleukin-8 and prostaglandin E(...Continue Reading

References

Aug 1, 1991·Journal of Clinical Periodontology·U ZappaM Espeland
Aug 1, 1995·Trends in Microbiology·J M Cavaillon
Jun 1, 1996·The Journal of Surgical Research·W M BowlingM P Callery
Aug 1, 1996·Journal of Periodontal Research·M WilsonB Henderson
Aug 26, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Okada, S Murakami
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M D LehnerT Hartung
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M MartinS M Michalek
Jan 10, 2002·Critical Care Medicine·Michael A West, Wyrta Heagy
Dec 6, 2002·Journal of Medical Microbiology·Brian HendersonJohn M Ward
May 10, 2003·Trends in Immunology·Mihai G NeteaBart Jan Kullberg
Jun 14, 2003·Journal of Endotoxin Research·Jean-Marc CavaillonMinou Adib-Conquy
Jul 15, 2003·Clinical Microbiology Reviews·Edwin S Van AmersfoortJohan Kuiper
Jul 16, 2003·Trends in Immunology·Taro Fukao, Shigeo Koyasu
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
Jun 24, 2004·European Journal of Immunology·Barry Kilmartin, Denis J Reen
Jan 25, 2005·Infection and Immunity·Manoj MuthukuruChristopher W Cutler
Sep 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Barbara K YozaCharles E McCall
Oct 19, 2006·Critical Care : the Official Journal of the Critical Care Forum·Jean-Marc Cavaillon, Minou Adib-Conquy
Mar 14, 2007·Acta Odontologica Scandinavica·Angélica R HannasLeo Tjäderhane

❮ Previous
Next ❯

Citations

Jul 5, 2012·PloS One·Qisheng PengMary Beth Humphrey
Aug 24, 2013·International Journal of Molecular Sciences·Marja T PöllänenRiikka Ihalin
Aug 18, 2010·Periodontology 2000·Sami A ZelkhaSalomon Amar
Jul 24, 2014·Molecular Oral Microbiology·J DeschnerM Nokhbehsaim
Nov 13, 2014·Molecular Oral Microbiology·S Amar, M Engelke

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.