Macrophage uptake of cylindrical microparticles investigated with correlative microscopy

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Clemens TschekaMarc Schneider

Abstract

Cylindrical particles offer the opportunity to develop controlled and sustained release systems for the respiratory tract. One reason is that macrophages can phagocyte such particles only from either of the two ends. We investigated the uptake behaviour of murine alveolar macrophages incubated with elongated submicron-structured particles. For that purpose, fluorescent model silica nanoparticles were interconnected with the biocompatible polysaccharide agarose, building up cylindrical particles within the pores of track-etched membranes. In contrast to common approaches we determined the uptake at different time points with scanning electron microscopy, fluorescence microscopy, and the combination of both techniques - correlative microscopy (CLEM). As a consequence, we could securely identify uptake events and observe in detail the engulfment of particles and confirm, that phagocytosis could only be observed from the tips of the cylinders. CLEM allowed a comparison of the uptake measured with different techniques at identical macrophages. Qualitative and quantitative evaluation of this cylindrical particle uptake showed substantial differences between fluorescence microscopy, electron microscopy and the combination of both (CLE...Continue Reading

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Citations

Jul 21, 2017·Advanced Healthcare Materials·Michael MöhwaldMarc Schneider
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Cecilia VelinoMichele Iafisco
Mar 9, 2021·Frontiers in Immunology·Maksim V BaranovGeert van den Bogaart
Nov 14, 2016·ACS Biomaterials Science & Engineering·Antonio G B CastroFang Yang
Sep 19, 2019·ACS Medicinal Chemistry Letters·Armira AzuarMariusz Skwarczynski

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