Macrophages are a source of extracellular adenosine deaminase-2 during inflammatory responses

Clinical and Experimental Immunology
B A Conlon, W R Law

Abstract

Serum activity of the adenosine deaminase (ADA) isozyme, ADA2, has been reported to be elevated during various disease states. Macrophages have been suggested as the cellular source of extracellular ADA activity because they are one of the only cell types in which intracellular ADA2 activity has been measured, but extracellular secretion has never been demonstrated. Rat primary peritoneal macrophages (PPMs) and peripheral blood monocytes (PBMs) were harvested and incubated for 18 h in RPMI supplemented with horse serum. PPM and PBM lysates were assayed for intracellular ADA activity (ammonia production). In vitro and in vivo extracellular ADA activities were measured in media and rat serum, respectively. Activity of ADA1 was confirmed by selective inhibition with erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA). ADA2 activity was inhibited by 2'-deoxycoformcin only, and was increased at a low pH (6.5). Activity of both ADA isozymes was found in PPMs and PBMs, and their media. In a separate group of rats, peritonitis was induced by ip insertion of 400 mg/kg caecal slurry. PPMs were harvested 24 h later and incubated for 18 h. In PPMs from rats with peritonitis both isozymes were elevated by a similar proportion. In contrast, media f...Continue Reading

References

Jun 1, 1991·Biochemical Pharmacology·J G Niedzwicki, D R Abernethy
Aug 1, 1988·Journal of Leukocyte Biology·D L LaskinJ D Laskin
Nov 15, 1986·Analytical Biochemistry·M J Danton, M S Coleman
Dec 15, 1971·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P C LeeP F Ma
Sep 1, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Ratech, R Hirschhorn
Sep 1, 1995·Clinical Immunology and Immunopathology·R Hirschhorn
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·M WakamiyaR E Kellems
Jun 1, 1995·Chest·C Gakis
Apr 1, 1996·The Journal of Laboratory and Clinical Medicine·T ShibagakiK Shimokata
Oct 23, 1997·The Journal of Biological Chemistry·M R DusingD A Wiginton
Jun 18, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·I Ben-Shooshan, A H Parola
Feb 24, 2001·Respirology : Official Journal of the Asian Pacific Society of Respirology·M GorgunerI Gorguner
Feb 27, 2001·Medicinal Research Reviews·G CristalliE Camaioni
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Simon AdaninWilliam R Law
Jun 18, 2002·European Journal of Pharmacology·Satyanarayan G BhatVickram Ramkumar
Jul 2, 2002·American Journal of Respiratory and Critical Care Medicine·Elliott S CohenSteven M Hollenberg
Apr 30, 2003·European Journal of Internal Medicine·Luis ValdésJosé Manuel Martínez Vázquez

❮ Previous
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Citations

Jul 30, 2014·Molecular and Cellular Biochemistry·Fátima Husein AbdallaCinthia Melazzo Andrade Mazzanti
Jan 6, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·F H AbdallaM B Moretto
Aug 24, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·V C PimentelM B Moretto
Oct 14, 2008·Pharmacology & Therapeutics·Luca AntonioliCorrado Blandizzi
Jul 17, 2013·Journal of Oral Rehabilitation·A YoshiharaH Miyazaki
Jun 17, 2014·Medicinal Research Reviews·Antoni CortésVicent Casadó
Aug 13, 2013·Toxicology and Applied Pharmacology·Carlos Eduardo LeiteAna Maria Oliveira Battastini
May 21, 2013·Biochemical and Biophysical Research Communications·Nehal M ElsherbinyGregory I Liou
Mar 13, 2014·Drug Discovery Today·Luca AntonioliGyörgy Haskó
Sep 24, 2014·Nature Reviews. Rheumatology·Fabio Martinon, Ivona Aksentijevich
Oct 20, 2015·Trends in Pharmacological Sciences·Mauro PerrettiTrinidad Montero-Melendez
Jul 27, 2006·The Journal of International Medical Research·A KafkasliR Laurini
Nov 21, 2007·Inflammatory Bowel Diseases·Luca AntonioliCorrado Blandizzi
May 29, 2007·Shock·William R LawJames D Ross
Jul 30, 2010·The Journal of Pharmacology and Experimental Therapeutics·Luca AntonioliCorrado Blandizzi
Jun 2, 2005·The Biochemical Journal·Andrey V Zavialov, Ake Engström
Aug 5, 2008·Journal of Perinatal Medicine·Salih TaşkinFeride Söylemez
May 23, 2020·International Journal of Molecular Sciences·Francisco Miguel Conesa-BuendíaAránzazu Mediero
May 20, 2011·European Journal of Gastroenterology & Hepatology·Irit MaorAlexandra Lavy
Dec 20, 2018·Immunological Reviews·Leen MoensIsabelle Meyts
Oct 3, 2019·Journal of Human Reproductive Sciences·Harsha PratapHanuman Srinivas Bheemanathi
Nov 30, 2019·Chemical Research in Toxicology·Jessica L Macer-WrightClare L Hawkins

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