Aug 21, 2012

Macrophages modulate the viability and growth of human mesenchymal stem cells

Journal of Cellular Biochemistry
Donald O FreytesGordana Vunjak-Novakovic

Abstract

Following myocardial infarction, tissue repair is mediated by the recruitment of monocytes and their subsequent differentiation into macrophages. Recent findings have revealed the dynamic changes in the presence of polarized macrophages with pro-inflammatory (M1) and anti-inflammatory (M2) properties during the early (acute) and late (chronic) stages of cardiac ischemia. Mesenchymal stem cells (MSCs) delivered into the injured myocardium as reparative cells are subjected to the effects of polarized macrophages and the inflammatory milieu. The present study investigated how cytokines and polarized macrophages associated with pro-inflammatory (M1) and anti-inflammatory (M2) responses affect the survival of MSCs. Human MSCs were studied using an in vitro platform with individual and combined M1 and M2 cytokines: IL-1β, IL-6, TNF-α, and IFN-γ (for M1), and IL-10, TGF-β1, TGF-β3, and VEGF (for M2). In addition, polarization molecules (M1: LPS and IFN-γ; M2: IL-4 and IL-13) and common chemokines (SDF-1 and MCP-1) found during inflammation were also studied. Indirect and direct co-cultures were conducted using M1 and M2 polarized human THP-1 monocytes. M2 macrophages and their associated cytokines supported the growth of hMSCs, while ...Continue Reading

Mentioned in this Paper

Ischemia
Stromal Cell-Derived Factor 1
Coculture Techniques
Recombinant Interleukin-10
Interleukin-1
Anti-Inflammatory Agents
VEGFA gene
Bone Marrow Stromal Cells
Monocyte Chemoattractant Protein-1
Myocardium

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