Macropinosome formation by tent pole ruffling in macrophages

The Journal of Cell Biology
Nicholas D CondonAdam A Wall

Abstract

Pathogen-mediated activation of macrophages arms innate immune responses that include enhanced surface ruffling and macropinocytosis for environmental sampling and receptor internalization and signaling. Activation of macrophages with bacterial lipopolysaccharide (LPS) generates prominent dorsal ruffles, which are precursors for macropinosomes. Very rapid, high-resolution imaging of live macrophages with lattice light sheet microscopy (LLSM) reveals new features and actions of dorsal ruffles, which redefine the process of macropinosome formation and closure. We offer a new model in which ruffles are erected and supported by F-actin tent poles that cross over and twist to constrict the forming macropinosomes. This process allows for formation of large macropinosomes induced by LPS. We further describe the enrichment of active Rab13 on tent pole ruffles and show that CRISPR deletion of Rab13 results in aberrant tent pole ruffles and blocks the formation of large LPS-induced macropinosomes. Based on the exquisite temporal and spatial resolution of LLSM, we can redefine the ruffling and macropinosome processes that underpin innate immune responses.

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Citations

Feb 1, 2019·Journal of Cell Science·Kan Etoh, Mitsunori Fukuda
May 8, 2019·Proceedings of the National Academy of Sciences of the United States of America·Nydia Tejeda-MuñozEdward M De Robertis
Jan 16, 2020·The Journal of Cell Biology·Sandra Sofía Edwards-JorqueraÁlvaro Glavic
Aug 4, 2020·Journal of Leukocyte Biology·Jennifer L Stow, Nicholas D Condon
Jan 31, 2019·Optics Express·Liang GaoBi-Chang Chen
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Thomas D WilliamsRobert R Kay
Jan 1, 2020·Journal of Cell Science·Lin LuoJennifer L Stow
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Adam A WallJennifer L Stow
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Joel A Swanson, Jason S King
Aug 7, 2020·Membranes·Xiao Peng LinPaul A Gleeson
Oct 12, 2018·The Journal of Cell Biology·Reuben V Philip, Rene E Harrison
Aug 4, 2020·Current Opinion in Cell Biology·Jennifer L StowAdam A Wall
Jan 20, 2020·Bio-protocol·Nicholas D CondonAdam A Wall
Mar 14, 2021·Nature Nanotechnology·Joshua J RennickRobert G Parton
Apr 14, 2021·Cellular Microbiology·Yuen-Yan ChangVirginie Stévenin
May 26, 2021·Current Biology : CB·Sivakami MylvaganamSergio Grinstein
Jun 16, 2021·The Journal of Cell Biology·Yvette W H Koh, Jennifer L Stow
Jun 25, 2021·The Journal of Cell Biology·Anh Hoang LeLaura M Machesky
Jun 28, 2021·Cells & Development·Robert R Kay
Aug 5, 2020·Current Biology : CB·Robert R Kay
Jul 2, 2021·Annual Review of Cell and Developmental Biology·Lauren V AlbrechtEdward M De Robertis

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Methods Mentioned

BETA
light sheet
GTPase
pulldowns
FRET
biosensors
transfections
biosensor
PCR
FCS
pull down

Software Mentioned

Fiji
ImageJ
GraphPad Prism
Excel
Amira
Janelia
Imaris
LLSM
Arivis Vision4D X64
Biosystems

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