Macroscopic streamer growths in acidic, metal-rich mine waters in north wales consist of novel and remarkably simple bacterial communities

Applied and Environmental Microbiology
Kevin B HallbergD Barrie Johnson

Abstract

The microbial composition of acid streamers (macroscopic biofilms) in acidic, metal-rich waters in two locations (an abandoned copper mine and a chalybeate spa) in north Wales was studied using cultivation-based and biomolecular techniques. Known chemolithotrophic and heterotrophic acidophiles were readily isolated from disrupted streamers, but they accounted for only <1 to 7% of the total microorganisms present. Fluorescent in situ hybridization (FISH) revealed that 80 to 90% of the microbes in both types of streamers were beta-Proteobacteria. Terminal restriction fragment length polymorphism analysis of the streamers suggested that a single bacterial species was dominant in the copper mine streamers, while two distinct bacteria (one of which was identical to the bacterium found in the copper mine streamers) accounted for about 90% of the streamers in the spa water. 16S rRNA gene clone libraries showed that the beta-proteobacterium found in both locations was closely related to a clone detected previously in acid mine drainage in California and that its closest characterized relatives were neutrophilic ammonium oxidizers. Using a modified isolation technique, this bacterium was isolated from the copper mine streamers and shown...Continue Reading

References

May 1, 1992·Applied and Environmental Microbiology·D B JohnsonM F Said
Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Jan 1, 1985·Applied and Environmental Microbiology·D J Reasoner, E E Geldreich
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 31, 2000·Applied and Environmental Microbiology·P L BondJ F Banfield
Jan 5, 2002·Advances in Applied Microbiology·K B Hallberg, D B Johnson
Aug 7, 2003·Applied and Environmental Microbiology·E González-TorilR Amils
Jun 9, 2004·FEMS Microbiology Letters·Ana I López-ArchillaPurificación López-García
Jul 1, 1987·Applied and Environmental Microbiology·D R Lovley, E J Phillips
May 1, 2003·FEMS Microbiology Ecology·Brett J Baker, Jillian F Banfield

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Citations

Jan 22, 2010·Analytical and Bioanalytical Chemistry·Thuro ArnoldNils Baumann
Aug 23, 2008·Extremophiles : Life Under Extreme Conditions·D Barrie JohnsonKevin B Hallberg
Dec 18, 2008·Extremophiles : Life Under Extreme Conditions·Welington I AlmeidaOrlando B Martins
Aug 17, 2010·Extremophiles : Life Under Extreme Conditions·Chunbo HaoHailiang Dong
Oct 16, 2012·Extremophiles : Life Under Extreme Conditions·Lukáš Falteisek, Ivan Cepička
Aug 22, 2013·Extremophiles : Life Under Extreme Conditions·A R LuchetaM R Lambais
Apr 14, 2010·Journal of Fluorescence·Sina BrockmannGert Bernhard
Aug 30, 2008·The ISME Journal·Suzanne C M HaaijerHuub J M Op den Camp
Jul 3, 2009·The ISME Journal·Katharina BesemerTom J Battin
Apr 2, 2010·Journal of the Royal Society, Interface·Roberto RusconiHoward A Stone
Jul 1, 2008·Applied and Environmental Microbiology·Dagmar Kock, Axel Schippers
Dec 3, 2008·Applied and Environmental Microbiology·Elke HeinzelMichael Schlömann
Dec 31, 2008·Applied and Environmental Microbiology·Sven C NeulingerWolf-Christian Dullo
Oct 26, 2010·Applied and Environmental Microbiology·Shipeng LuKirsten Küsel
Mar 17, 2010·Applied and Environmental Microbiology·Yongqin JiaoMichael P Thelen
Feb 10, 2009·Applied and Environmental Microbiology·Brett J BakerJillian F Banfield
Apr 22, 2008·Applied and Environmental Microbiology·Monica O MendezRaina M Maier
Feb 14, 2013·Genome Announcements·Luis E Servín-GarcidueñasEsperanza Martínez-Romero
Aug 21, 2013·Journal of Microbiological Methods·Judith S TischlerMichael Schlömann
Jan 1, 2015·Microorganisms·María Sofía UrbietaEdgardo Rubén Donati
Jun 15, 2011·The Science of the Total Environment·E Krawczyk-BärschU Zimmermann
Aug 30, 2011·Fungal Biology·Sara L HollandSimon V Avery
Feb 28, 2016·Current Opinion in Biotechnology·Lin-xing ChenWen-sheng Shu
Jun 13, 2009·Environmental Microbiology·Sibylle ZieglerJohannes Gescher

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