Mactinin treatment promotes wound-healing-associated inflammation in urokinase knockout mice

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
S LuikartTheodore Oegema

Abstract

Mactinin, a 31 kDa fragment from the amino-terminal end of alpha-actinin, is chemotactic for monocytes and can promote monocyte/macrophage maturation. Macrophages are essential for wound healing, in which they play key roles in debridement, angiogenesis, fibroblast proliferation, and collagen metabolism. We have previously determined that urokinase is necessary to form mactinin from extracellular alpha-actinin, which may be present at sites of inflammation as a result of cell movement. Thus, urokinase knockout mice are unable to form mactinin and therefore are an ideal model to study mactinin's effects on wound healing. Saline- and mactinin-treated wounds were analyzed in a subcutaneous sponge wound model in both wild-type and urokinase knockout mice. The wounded urokinase knockout mice had markedly decreased leukocyte infiltration compared with wounded wild-type mice. In addition, production of the proinflammatory cytokine, interleukin-12, and of collagen was also decreased in knockouts. Treatment of knockout mice with mactinin resulted in leukocyte infiltration numbers, interleukin-12 levels, and hydroxyproline measurements similar to those in wild-type mice. The results suggest that impaired wound healing in urokinase-defici...Continue Reading

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Citations

Sep 6, 2007·The Journal of Cell Biology·Mònica SuelvesPura Muñoz-Cánoves
Jul 29, 2008·International Journal of Cardiology·Jessica M LambertMerry L Lindsey
Mar 14, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Eun-Sook ParkKyun-Hwan Kim
Sep 9, 2010·Cell Communication and Signaling : CCS·Lido Calorini, Francesca Bianchini
Jan 30, 2010·PloS One·Jamila Khallou-LaschetGiuseppina Caligiuri
Aug 3, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Dmitri V PechkovskyGernot Zissel
Apr 12, 2008·The American Journal of Pathology·Glenda TrujilloCory M Hogaboam
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Feb 1, 2011·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Xiao-jun ZhangDavid N Herndon
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Scott C BryerTimothy J Koh

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