MagC is a NplC/P60-like member of the α-2-macroglobulin Mag complex of Pseudomonas aeruginosa that interacts with peptidoglycan.

FEBS Letters
Samira ZouhirPauline Macheboeuf

Abstract

Bacterial α-2 macroglobulins (A2Ms) structurally resemble the large spectrum protease inhibitors of the eukaryotic immune system. In Pseudomonas aeruginosa, MagD acts as an A2M and is expressed within a six-gene operon encoding the MagA-F proteins. In this work, we employ isothermal calorimetry (ITC), analytical ultracentrifugation (AUC), and X-ray crystallography to investigate the function of MagC and show that MagC associates with the macroglobulin complex and with the peptidoglycan (PG). However, the catalytic residues of MagC display an inactive conformation that could suggest that it binds to PG but does not degrade it. We hypothesize that MagC could serve as an anchor between the MagD macroglobulin and the PG and could provide stabilization and/or regulation for the entire complex.

References

Jan 1, 1992·Electron Microscopy Reviews·E DelainF Van Leuven
Dec 1, 1972·Bacteriological Reviews·K H Schleifer, O Kandler
Apr 5, 2001·Trends in Immunology·P B Armstrong
May 27, 2003·Trends in Biochemical Sciences·Daniel J RigdenMichael Y Galperin
May 27, 2003·Trends in Biochemical Sciences·Alex Bateman, Neil D Rawlings
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Mar 23, 2006·Acta Crystallographica. Section D, Biological Crystallography·Jay Painter, Ethan A Merritt
Jan 16, 2008·FEMS Microbiology Reviews·Waldemar VollmerMiguel A de Pedro
Apr 9, 2008·Journal of the American Chemical Society·Thomas KernJean-Pierre Simorre
Jan 1, 1997·Methods in Enzymology·A T Brünger
Jul 5, 2008·Nature Protocols·Gerrit LangerAnastassis Perrakis
Aug 18, 2009·Nature Methods·Dayté D RodríguezIsabel Usón
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
May 22, 2010·Molecular Microbiology·Marie-Stéphanie AschtgenEric Cascales
Aug 10, 2010·Acta Crystallographica. Section D, Biological Crystallography·Frank ZuckerEthan A Merritt
Oct 15, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Qingping XuIan A Wilson
Oct 30, 2010·Current Opinion in Structural Biology·Pierre-Jean MatteïAndréa Dessen
Feb 1, 2011·FEMS Microbiology Letters·Edie M Scheurwater, Lori L Burrows
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Garib N MurshudovAlexei A Vagin
Oct 4, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jeong Soon ParkHye-Yeon Kim
Oct 23, 2012·Journal of Cellular Physiology·Ahmed A RehmanFahim H Khan
Aug 8, 2013·MBio·Mylène Robert-GenthonIna Attrée
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Sep 16, 2014·Nature Communications·Steve G Wong, Andréa Dessen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.