Mage-b vaccine delivered by recombinant Listeria monocytogenes is highly effective against breast cancer metastases.

British Journal of Cancer
S H KimC Gravekamp

Abstract

New therapies are needed that target breast cancer metastases. In previous studies, we have shown that vaccination with pcDNA3.1-Mage-b DNA vaccine is effective against breast cancer metastases. In the study presented here, we have further enhanced the efficacy of Mage-b vaccination through the improved delivery of the vaccine using recombinant Listeria monocytogenes (LM). Three overlapping fragments of Mage-b as well as the complete protein-encoding region of Mage-b have been expressed as a fusion protein with a truncated non-cytolytic form of listeriolysin O (LLO) in recombinant LM. These different Mage-b vaccine strains were preventively tested for their efficacy against breast cancer metastases in a syngeneic mouse tumour model 4T1. The LM-LLO-Mage-b/2nd, expressing position 311-660 of the cDNA of Mage-b, was the most effective vaccine strain against metastases in the 4T1 mouse breast tumour model. Vaccination with LM-LLO-Mage-b/2nd dramatically reduced the number of metastases by 96% compared with the saline group and by 88% compared with the vector control group (LM-LLO), and this correlated with strong Mage-b-specific CD8 T-cell responses in the spleen, after restimulation with Mage-b. However, no effect of LM-LLO-Mage-b...Continue Reading

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Citations

Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Wilber Quispe-TintayaClaudia Gravekamp
Oct 16, 2013·Expert Review of Vaccines·Bertrand ToussaintAudrey Le Gouëllec
Mar 5, 2016·Oncoimmunology·Dinesh ChandraClaudia Gravekamp
Nov 21, 2015·Oncoimmunology·Claudia Gravekamp, Dinesh Chandra
Dec 9, 2014·Human Vaccines & Immunotherapeutics·Lori Cory, Christina Chu
Apr 7, 2011·Bioengineered Bugs·Chwanrow K BabanMark Tangney
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Mar 5, 2009·Cancer Immunology, Immunotherapy : CII·Claudia Gravekamp
Jun 23, 2009·Expert Review of Vaccines·Barbara J Mann, Nicole M Ark
Jun 8, 2017·Gynecologic Oncology Research and Practice·Brett A MilesHoward P Safran
Aug 22, 2017·Expert Opinion on Biological Therapy·Azam BolhassaniSepehr Soleymani
Sep 17, 2020·Oncoimmunology·Julie Le NaourGuido Kroemer
Nov 8, 2011·Cancer Immunology, Immunotherapy : CII·Laurence M WoodYvonne Paterson
Dec 17, 2011·Journal of Neuro-oncology·Adam M SonabendRichard C E Anderson
May 6, 2020·ImmunoTargets and Therapy·Tanvir F KabirAman Chauhan
May 20, 2016·Clinical & Translational Immunology·Leandro J CarreñoSteven A Porcelli
Jun 11, 2014·Cancer Immunology Research·Dinesh ChandraClaudia Gravekamp

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Methods Mentioned

BETA
surgical resection
biopsies
PCR
ELISA

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