Magic-angle spinning NMR of intact bacteriophages: insights into the capsid, DNA and their interface

Journal of Magnetic Resonance
Gili AbramovAmir Goldbourt

Abstract

Bacteriophages are viruses that infect bacteria. They are complex macromolecular assemblies, which are composed of multiple protein subunits that protect genomic material and deliver it to specific hosts. Various biophysical techniques have been used to characterize their structure in order to unravel phage morphogenesis. Yet, most bacteriophages are non-crystalline and have very high molecular weights, in the order of tens of MegaDaltons. Therefore, complete atomic-resolution characterization on such systems that encompass both capsid and DNA is scarce. In this perspective article we demonstrate how magic-angle spinning solid-state NMR has and is used to characterize in detail bacteriophage viruses, including filamentous and icosahedral phage. We discuss the process of sample preparation, spectral assignment of both capsid and DNA and the use of chemical shifts and dipolar couplings to probe the capsid-DNA interface, describe capsid structure and dynamics and extract structural differences between viruses.

References

Nov 5, 1976·Journal of Molecular Biology·E J WachtelD A Marvin
Nov 28, 1989·Biochemistry·R A SantosG S Harbison
Feb 10, 1987·Biochemistry·L A ColnagoS J Opella
Nov 5, 1997·Cell·M E CerritelliA C Steven
Jun 19, 1998·Current Opinion in Structural Biology·D A Marvin
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Feb 10, 2000·Acta Crystallographica. Section D, Biological Crystallography·L C WelshD A Marvin
Sep 7, 2001·Annual Review of Microbiology·W C Summers
Mar 23, 2002·Protein Science : a Publication of the Protein Society·Yunjun Wang, Oleg Jardetzky
Jul 16, 2002·Infection and Immunity·Patrick L Wagner, Matthew K Waldor
Mar 6, 2003·Nature Materials·Rajesh R NaikMorley O Stone
May 1, 1952·The Journal of General Physiology·A D HERSHEY, M CHASE
Dec 30, 1961·Nature·F H CRICKR J WATTS-TOBIN
May 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·I L IvanovskaG J L Wuite
May 27, 2004·Journal of the American Chemical Society·Tatyana I IgumenovaA Joshua Wand
Jun 10, 2004·Journal of the American Chemical Society·Corey R MorcombeKurt W Zilm
Nov 18, 2004·Journal of Bacteriology·Jeremy S WebbStaffan Kjelleberg
Feb 9, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Kirstin R Purdy, Seth Fraden
Mar 3, 2005·Protein Science : a Publication of the Protein Society·David S ThiriotStanley J Opella
Nov 10, 2005·Chemical Reviews·John W Kehoe, Brian K Kay
Dec 21, 2005·Applied Microbiology and Biotechnology·Matthias Paschke
May 9, 2006·Current Opinion in Chemical Biology·Anna Merzlyak, Seung-Wuk Lee
Jul 18, 2006·Nucleic Acids Research·Mark V BerjanskiiDavid S Wishart
Feb 7, 2007·Journal of the American Chemical Society·Amir GoldbourtAnn E McDermott
Jun 19, 2007·Biophysical Journal·C Rebecca LockerStephen C Harvey
Sep 29, 2007·Journal of Magnetic Resonance·Amir GoldbourtAnn E McDermott
Apr 9, 2008·Annual Review of Physical Chemistry·Stanley J OpellaSang Ho Park
Jul 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Justin L LorieauAnn E McDermott
Aug 16, 2008·Journal of Molecular Biology·Tsyr-Yan Yu, Jacob Schaefer
Sep 29, 2009·Journal of Magnetic Resonance·Fabian V FilippStanley J Opella
Oct 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rhiju DasDavid Baker
Jan 19, 2010·Biochemistry·Masamichi TsuboiGeorge J Thomas
Jul 17, 2010·Progress in Nuclear Magnetic Resonance Spectroscopy·Sik Lok Lam, Lai Man Chi
Aug 26, 2010·The Journal of Biological Chemistry·Amir GoldbourtAnn E McDermott
Oct 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ansgar B SiemerAnn E McDermott
Nov 18, 2010·European Biophysics Journal : EBJ·S K StrausD A Marvin

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Citations

Jun 24, 2017·Angewandte Chemie·Maximilian ZinkeAdam Lange
Mar 16, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Lauriane LecoqAnja Böckmann
Aug 10, 2019·Frontiers in Molecular Biosciences·Lauriane LecoqAnja Böckmann
Feb 17, 2018·Biomolecular NMR Assignments·Lauriane LecoqAnja Böckmann
Jun 19, 2018·Emerging Topics in Life Sciences·Patrick C A van der Wel
May 18, 2020·AAPS PharmSciTech·Yajie ZhangDebadyuti Ghosh
Nov 30, 2019·Progress in Nuclear Magnetic Resonance Spectroscopy·Amir Goldbourt
Sep 30, 2020·Viruses·Lauriane LecoqAnja Böckmann
Aug 2, 2017·The Journal of Physical Chemistry. B·Linda CerofoliniClaudio Luchinat

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