Magnesium deficiency affects mammary epithelial cell proliferation: involvement of oxidative stress

Nutrition and Cancer
Federica I WolfJeanette A M Maier

Abstract

Low Mg availability reversibly inhibited the growth of mammary epithelial HC11 cells by increasing the number of cells in the G0/G1 phase of the cell cycle. Because low Mg has been reported to promote oxidative reactions, we considered that low Mg-dependent growth arrest was mediated by oxidative stress. Surprisingly, both dichlorofluorescein-detectable reactive oxygen species and hydrogen peroxide-induced oxidative DNA damage were found to be lower in cells cultured in low Mg than in cells grown under control or high-Mg conditions. Gene expression profiling of low- and high-Mg cells showed the modulation of several genes, some regulating cell proliferation. In addition, low Mg cells also displayed overexpression of glutathione S-transferase (GST), leading to increased enzymatic activity. Of note, GST has been shown to modulate cell growth; therefore, we suggest that in low-Mg cells, GST upregulation might have a dual role in protecting against oxidative stress and in modulating cell proliferation.

Citations

Aug 6, 2013·Nutrients·Sara CastiglioniJeanette A M Maier
Jun 29, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Colleen G Le PrellKevin K Ohlemiller
Dec 22, 2015·Environmental Science and Pollution Research International·Tong LiuCheng Zhang
Feb 11, 2009·Cancer Treatment Reviews·Federica I WolfJeanette A M Maier
Sep 1, 2015·Biochimica Et Biophysica Acta·Yutaka ShindoKotaro Oka
Jun 8, 2012·Clinical Science·Federica I Wolf, Valentina Trapani
May 17, 2012·The Biochemical Journal·Hsiang-Chin ChenLoren W Runnels
Jan 6, 2018·International Journal of Molecular Sciences·Donogh MaguirePaul G Shiels
Jun 19, 2018·Nutrients·Francesca LuongoValentina Trapani
Nov 18, 2016·BioMedicine·Anastasia A ZheltovaAlexander A Spasov
Nov 19, 2017·Analytical and Bioanalytical Chemistry·Emil MalucelliStefano Iotti
Sep 8, 2019·Cell Calcium·Valentina Trapani, Federica I Wolf
Jun 7, 2011·Archives of Biochemistry and Biophysics·Andrea M P Romani

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